Transfection Reagents
Transfection Reagents
Cell Applications Inc CYTOFECT ENDOTHELIAL CELL KIT
Transfection Reagent Kit for human endothelial cells and animal endothelial cells
Cell Applications Inc Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns
Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns
Cell Applications Inc Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns
Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns
Cell Applications Inc Cryopreserved Epidermal Keratinocytes (HEK), Neonatal
Cryopreserved Epidermal Keratinocytes (HEK), Neonatal
Cell Applications Inc FIBROBLAST TRANSFECTION KIT
Cytofect™ Fibroblast Transfection Kit: 250 x 24-Well Rxns
Cell Applications Inc Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns
Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns
Lonza Walkersville SF Cell Line 96-well Nucleofector Kit
The SF Cell Line 96-well Nucleofector Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:2.25 mL SF Cell Line Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)1 x 96-well Nucleocuvette Plate (20 μL)
Lonza Walkersville P3 Primary Cell 384-well Nucleofector Kit
The P3 Primary Cell 384-well Nucleofector Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT Nucleofector System.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionSeamless integration into automated liquid handling environmentsConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers down to 2 x 10⁴ primary cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:22.5 mL P3 Primary Cell Nucleofector Solution5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 384-well Nucleocuvette Plate (20 μl)
Lonza Walkersville P3 Primary Cell 96-well Nucleofector Kit
The P3 Primary Cell 96-well Nucleofector Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies for primary cellsCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)1 x 96-well Nucleocuvette Plate (20 μl)
Lonza Walkersville SG Cell Line 96-well Nucleofector Kit
The SG Cell Line 96-well Nucleofector Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:2.25 mL SG Cell Line Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)1 x 96-well Nucleocuvette Plate (20 μl)
Lonza Walkersville P3 Primary Cell 4D-Nucleofector LV Kit L
The P3 Primary Cell 4D-Nucleofector LV Kit L is suited for larger scale transfection of primary cells in the 1 mL Nucleocuvette Cartridge format when working with the 4D-Nucleofector LV Unit. With the 1 mL Nucleocuvette Cartridge a fixed volume of 1 mL can be transfected. The cartridge is manually filled via a sterile injection port.Applications:For large scale transfection of up to 1 x 10⁸ cellsEx-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)Generation of large numbers of transiently modified primary cells for cell-based assaysContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 1 mL Nucleocuvette Cartridge
Lonza Walkersville SF Cell Line 4D-Nucleofector X Kit L
The SF Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput Applications Content:2 x 0.675 mL SF Cell Line Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessel12 x Single use pipettes
Lonza Walkersville SF Cell Line 4D-Nucleofector X Kit L
The SF Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System (including the , 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applications Content:2.25 mL SF Cell Line Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)24 x 100 µL Nucleocuvette Vessel24 x Single use pipettes
Lonza Walkersville P3 Primary Cell 4D-Nucleofector LV Kit XL
The P3 Primary Cell 4D-Nucleofector LV Kit XL* is suited for larger scale transfection of primary cells in the LV Nucleocuvette Cartridge format when working with the 4D-Nucleofector LV Unit. With the LV Nucleocuvette Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.Applications:For large scale transfection of up to 1 x 10⁹ cellsEx-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)Generation of large numbers of transiently modified primary cells for cell-based assaysContent:22.5 mL P3 Primary Cell Nucleofector Solution5 mL Supplement 1,1 x LV Nucleocuvette Cartridge2 x 4D-Nucleofector LV Reservoir
Lonza Walkersville P3 Primary Cell 4D-Nucleofector X Kit S
The P3 Primary Cell 4D-Nucleofector X Kit S is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applications Optimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL P3 Primary Cell Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well Nucleocuvette Strips (20 μl)
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