Oligonucleotides
Oligonucleotides
New England Biolabs, Inc. Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) - 8000 units
Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows. Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. At temperatures above 45°C, the aptamer rapidly releases from the enzyme with no special activation step needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5' to 3' DNA polymerase activity and strong strand-displacement activity but lacks 5' to 3' exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
New England Biolabs, Inc. EnGen SpRY Cas9 - 500 pmol
EnGen SpRY Cas9 from Streptococcus pyogenes is an engineered, RNA-guided, DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA (dsDNA).
New England Biolabs, Inc. LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG - 96 rxns
The LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage prior to use. The mix contains all necessary components for one-step RT-qPCR except primers, probe(s), and RNA sample. It can be reconstituted by simply adding water to a 2-4X concentration, allowing for increased sample input. Performance in multiplexing applications has been optimized, with sensitive, linear detection achieved for up to 5 targets across a range of inputs. It is available in 2 x 60 reaction glass vials or a 96-well plate (1 reaction per well).
New England Biolabs, Inc. LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG) - 100 rxns
The LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage prior to use. It is a simple, one-step solution for real-time detection of LAMP/RT-LAMP reactions via fluorescence. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers and a strand-displacing DNA polymerase. Featuring a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution, this mix offers improved performance. LAMP Fluorescent Dye at 0.5X concentration is included to enable detection of amplification via real time fluorescence. Antarctic Thermolabile UDG and dUTP reduce the risk of carryover contamination between reactions. It is available in 2 x 50 reaction glass vials or a 96-well plate (1 reaction per well).
New England Biolabs, Inc. NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 3) – 384 reactions
NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. Unique Dual Index (UDI) primer pairs enable the identification and mitigation of the impacts of index hopping when using a patterened flow cell. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 96 pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal. Additional NEBNext Multiplex Oligos products are available for different indexing strategies.
New England Biolabs, Inc. NEBNext Multiplex Oligos for Illumina (Index Primers Set 3) – 96 reactions
NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. Additional NEBNext Multiplex Oligos products are available for different indexing strategies.
New England Biolabs, Inc. NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) – 96 reactions
NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. Unique Dual Index (UDI) primer pairs enable the identification and mitigation of the impacts of index hopping when using a patterened flow cell. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 96 pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal. Additional NEBNext Multiplex Oligos products are available for different indexing strategies.
New England Biolabs, Inc. NEBNext Enzymatic 5hmC-seq Kit 24 rxns
The NEBNext Enzymatic 5hmC-seq Kit specifically detects 5hmC sites. The kit includes NEBNext Ultra II library prep reagents, and 5hmC is detected using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC after Illumina sequencing. E5hmC-seq data can also be subtracted from EM-seq data, allowing determination of the precise location of individual 5mC and 5hmC sites. The kit includes the E5hmC-seq adaptor and index primers are supplied separately.
New England Biolabs, Inc. NEBNext UltraExpress™ FS DNA Library Prep Kit - 24 rxns
The NEBNext UltraExpress FS DNA Library Prep Kit is the latest generation of NEBNext DNA library prep, with a fast, streamlined workflow, including a fragmentation step. In under 2 hours, the protocol enables the creation of high yield, high quality libraries, from a broad input amount range of intact DNA, while generating less plastic waste. Further, with simplicity at the forefront, the kit features a single protocol for all input amounts, making NEBNext UltraExpress DNA Library Prep well suited for all throughputs.
New England Biolabs, Inc. NEBNext Enzymatic 5hmC-seq Conversion Module 96 rxns
The NEBNext Enzymatic 5hmC-seq Conversion Module contains the reagents required to enzymatically modify and enable specific detection of 5-hydroxymethylcytosine (5hmC). The user-friendly workflow enables 5hmC identification from 0.1 – 200 ng of input DNA.
New England Biolabs, Inc. NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) – 96 reactions
NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing. Additional NEBNext Multiplex Oligos products are available for different indexing strategies.
New England Biolabs, Inc. NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) – 24 reactions
NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. Additional NEBNext Multiplex Oligos products are available for different indexing strategies.
New England Biolabs, Inc. Monarch Spin PCR & DNA Cleanup Kit - 250 preps
The Monarch Spin PCR & DNA Cleanup Kit (5 μg), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 μl, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps.
New England Biolabs, Inc. Monarch Spin PCR & DNA Cleanup Kit - 50 preps
The Monarch Spin PCR & DNA Cleanup Kit (5 μg), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 μl, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps.
New England Biolabs, Inc. NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) – 24 reactions
NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina sequencing and includes the NEBNext EM-seq Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification. Unique Dual Index (UDI) primer pairs enable the detection and mitigation of index hopping as seen on patterened flow cells. The index primers are provided as pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal, or in individual vials. Although optimized for the EM-seq workflow, the NEBNext EM-seq Adaptor is also compatible with bisulfite sequencing workflows. These components are also included in the NEBNext Enzymatic Methyl-seq Kit. Additional sets of NEBNext Multiplex Oligos are available for different indexing strategies.