Protein Purification Reagents and Kits
Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Human Cord Blood CD34 Positive Selection Kit II, For processing: 1000 mL cord blood processed
Complete kit for CD34+ cells from cord blood
STEMCELL Technologies EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit, For processing: 2 x 10^9 cells
The EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit is designed to isolate CD4+CD127lowCD49d- cells from fresh peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells, CD127high, CD49d+ cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Human B Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Enrichment Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-B cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For even faster cell isolations, we recommend the new EasySep™ Human B Cell Isolation Kit (17954) (/products/easysep-human-b-cell-isolation-kit.html), which isolates cells in just 9 minutes.
STEMCELL Technologies RosetteSep™ Human Multiple Myeloma Cell Enrichment Cocktail, For processing: 200 mL bone marrow processed
The RosetteSep™ Human Multiple Myeloma Cell Enrichment Cocktail is designed to enrich multiple myeloma cells (B cell and plasma cells) from fresh bone marrow aspirates by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD33, CD41, CD45RA, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified multiple myeloma cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit is designed to isolate CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.This product replaces the EasySep™ Human Whole Blood CD19 Positive Selection Kit (Catalog #18084) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human CD45 Depletion Kit II, For processing: 2 x 10^9 cells
The EasySep™ Human CD45 Depletion Kit II is designed to deplete CD45+ cells from fresh or previously frozen human peripheral blood mononuclear cells. Unwanted cells are targeted with antibody complexes recognizing CD45 and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off. The CD45 antigen is expressed on all hematopoietic cells except erythrocytes and platelets.This product replaces the EasySep™ Human CD45 Depletion Kit (Catalog #18259) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail, For processing: 200 mL bone marrow processed
The RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail is designed to isolate mesenchymal stem cells from fresh bone marrow by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD14, CD19, CD38, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified mesenchymal stem cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RosetteSep™ Human CD45 Depletion Cocktail, For processing: 200 mL whole blood processed
The RosetteSep™ Human CD45 Depletion Cocktail is designed to enrich epithelial circulating tumor cells (CTCs) from whole blood by depleting CD45+ cells. Unwanted cells are targeted for depletion with Tetrameric Antibody Complexes recognizing CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified epithelial tumor cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies EasySep™ Dead Cell Removal (Annexin V) Kit, For processing: 1 x 10^9 cells
The EasySep™ Dead Cell Removal (Annexin V) kit is designed to deplete apoptotic (Annexin V+) cells from cell culture or tissue preparations by immunomagnetic negative selection. Unwanted cells are labeled with Annexin V, antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are simply poured off into a new tube. Annexin V binds phosphatidylserine on the outer leaflet of the cell membrane during apoptosis.
STEMCELL Technologies RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36, For processing: 40 mL whole blood processed
The RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36 is designed to enrich circulating epithelial tumor cells from fresh whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD16, CD19, CD36, CD38, CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified tumor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.This cocktail is compatible with small-cell carcinoma samples, including lung cancer samples. If you are working with breast cancer samples, use the RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD56 (Catalog #15137).
STEMCELL Technologies EasySep™ Human CD34 Positive Selection Kit II, For processing: 5 x 10^9 cells
The EasySep™ Human CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh or previously frozen bone marrow or mobilized peripheral blood mononuclear cells, or from previously frozen cord blood mononuclear cells, or from hESC/hiPSC-derived hematopoietic cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human CD4 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD4 Positive Selection Kit II is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing CD4 and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human CD4 Positive Selection Kit (Catalog #18052) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human Gamma/Delta T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Gamma/Delta T Cell Isolation Kit is designed to isolate gamma/delta T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-gamma/delta T cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube. To obtain a custom kit optimized for culture-expanded PBMCs, contact STEMCELL Technologies’ Technical Support at techsupport@stemcell.com.
STEMCELL Technologies Easy 250 EasySep™ Magnet, For isolating: 1.25 x 10^10 cells
Scale up your manual cell isolations from large-volume samples, such as leukopaks and whole blood. Rather than splitting your cell suspension and performing multiple rounds of isolations, with the Easy 250 EasySep™ magnet you can process up to 225 mL and 1.25 x 1010 cells in a single isolation.Use the Easy 250 EasySep™ magnet with a standard T-75 cm2 cell culture flask and EasySep™ reagents to separate cells from large-volume samples without the use of columns. With its strong, high-gradient internal magnetic field, the magnet isolates cells labeled with magnetic EasySep™ particles, allowing you to simply pipette out the unlabeled cell fraction and obtain cells that are immediately ready for downstream applications. Save time and obtain highly purified cells with EasySep™ column-free technology. Learn more about how EasySep™ technology works or get an overview of our other magnets on our EasySep™ Cell Separation Magnets page.
STEMCELL Technologies RoboSep™ Mouse Pan-Naïve T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Pan-Naïve T Cell Isolation Kit is designed to isolate T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-naïve T cells (CD11b, CD19, CD24, CD25, CD44, CD45R/B220, CD49b, TER119) and streptavidin-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.Click here (/easysep-15-minute-kits) to learn about our next-generation EasySep™ mouse cell isolation kits, featuring RapidSphere™ technology.