Amino Acid Analysis Reagents and Accessories
Amino Acid Analysis Reagents and Accessories
Enzo Life Sciences Adipogenesis assay kit (5x24wells)
The Adipogenesis Assay Kit provides all the reagents needed to induce and detect adipogenesis. Optimized Differentiation, Insulin, and Enhancer solutions ensure maximal adipocyte induction. The Adipogenesis Dye allows for visual confirmation of differentiation and can also be easily extracted allowing measurement using a spectrophotometer or microplate reader. Applications: Colorimetric detection .
Enzo Life Sciences CYTO-ID Autophagy detection kit (1x50tests)
A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer (CAT) dye that rapidly partitions into cells in a similar manner as drugs that induce phospholipidosis. Careful selection of titratable functional moieties on the dye prevents its accumulation within lysosomes, but enables labeling of vacuoles associated with the autophagy pathway. Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection, HTS. Contents: CYTO-ID Green Detection Reagent. Hoechst 33342 Nuclear Stain. Autophagy Inducer (Rapamycin). Chloroquine Control. 10X Assay Buffer
Enzo Life Sciences MITO-ID Membrane potential detection kit (100tests)
Cell-based assays for evaluating the functional status of mitochondria are emerging as useful tools for elucidating the role of mitochondrial activity in drug-induced toxicity, the apoptosis cascade and other cellular and biochemical processes. The loss of the mitochondrial membrane potential (MMP) is often associated with early stages of apoptosis. The collapse of MMP coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. MITO-ID Membrane Potential Detection Kit measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Enzo Life Sciences PROTEOSTAT PDI assay kit (50tests)
The PROTEOSTAT PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT PDI detection dye. Relative to analogous turbidimetric assays of PDI activity, the fluorescence-based assay provides a vastly improved assay signal window, improved lower detection limit, and superior Z’-score (>0.8). Intra-plate and inter-plate CVs using the assay are typically 3-5%. Human recombinant PDI enzyme (EC 5.3.4.1) and the PDI inhibitor, bacitracin, are included in the kit, as well as all reagents necessary for monitoring changes in PDI activity. Alternative name: Protein disulfide isomerase. Applications: Fluorescent detection .
Enzo Life Sciences 27-Hydroxycholesterol (5mg). CAS: 20380-11-4
27-Hydroxycholesterol (27-HC) is a metabolite resulting from the action of sterol 27-Hydroxylase on cholesterol. It is an endogenous selective estrogen receptor modulator and ligand for the liver X receptor (LXR). 27-Hydroxycholesterol plays a role in Alzheimer's disease, oxidative stress, metabolism, cell-signaling, and heart disease. Alternative name: 5,25R-Cholesten-3β,26-diol, (25R)-26-Hydroxycholesterol. Purity: ≥98% (HPLC). Solubility: Soluble in DMSO (up to 10 mg/ml with warming) or in ethanol (up to 10 mg/ml). Insoluble in water. Long Term Storage: -20°C.
Enzo Life Sciences ROS-ID Hypoxia/Oxidative stress detection kit (125tests)
Enzo Life Sciences’ ROS-ID Hypoxia/Oxidative stress detection kit is designed for functional detection of hypoxia and oxidative stress levels in live cells (both suspension and adherent) using fluorescent microscopy or flow cytometry. This kit includes fluorogenic probes for hypoxia (red) and for oxidative stress levels (green) as two major components. The Hypoxia (Red) dye takes advantage of the nitroreductase activity present in hypoxic cells by converting the Nitro group to hydroxylamine (NHOH) and amino (NH2) and releasing the fluorescent probe. The generated fluorescent products can be visualized using a wide-field fluorescence microscope equipped with standard fluorescein (490/525 nm) and Texas Red (596/670 nm) filters, confocal microscopy, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Enzo Life Sciences MITO-ID Membrane potential detection kit (25tests)
Cell-based assays for evaluating the functional status of mitochondria are emerging as useful tools for elucidating the role of mitochondrial activity in drug-induced toxicity, the apoptosis cascade and other cellular and biochemical processes. The loss of the mitochondrial membrane potential (MMP) is often associated with early stages of apoptosis. The collapse of MMP coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. MITO-ID Membrane Potential Detection Kit measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Enzo Life Sciences PROTEOSTAT Thermal shift stability assay kit (1x100tests)
Accelerated screening for protein stability as a function of pH, ionic strength, and concentration. The PROTEOSTAT Thermal shift stability assay kit includes a fluorescent dye which detects protein aggregation, so it can be used to monitor protein stability under systematic thermal stress conditions. From the thermal shift assay, a temperature at which the bulk of the protein becomes aggregated can readily be identified. The aggregation temperature is an indicator of protein stability and can be used to optimize conditions that minimize protein aggregation as well as to identify ligands that bind and confer structural stability to a protein of interest. Use the related PROTEOSTAT Protein Refolding and Aggregation Sensing Kit (Prod. No. ENZ-51040) to identify optimal protein refolding conditions. Conditions that increase the aggregation temperature, increase the stability of the protein. Contents: PROTEOSTAT TS Detection Reagent. β-Lactoglobulin control. 10X Assay Buffer
Enzo Life Sciences MITO-ID Green detection kit (100 tests)
Photostable, non-toxic and selective mitochondrial dye that stains regardless of membrane potential. MITO-ID Green Detection Kit contains a proprietary membrane-permeable mitochondria-selective dye suitable for use with live-, detergent-permeabilized- and aldehyde-fixed-cells. Unlike conventional dyes (such as DiOC6(3), JC-1, rhodamine 123 and tetramethylrhodamine ethyl ester) MITO-ID Green dye highlights mitochondria regardless of their energetic state. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes as well as High Content Screening (HCS) platforms. The kit is useful for assessing mitochondrial morphology changes, estimating mitochondrial mass and co-localizing RFP-tagged proteins to the mitochondrial compartment. Applications: Fluorescence microscopy, Fluorescent detection.
Enzo Life Sciences MITO-ID Red detection kit (GFP-CERTIFIED) (100tests)
MITO-ID Red Detection Kit (GFP-CERTIFIED) contains a proprietary membrane-permeable mitochondria-selective dye suitable for use with live-, detergent-permeabilized- and even aldehyde-fixed-cells. Unlike conventional dyes, such as DiOC6(3), JC-1, rhodamine 123 and tetramethylrhodamine ethyl ester, MITO-ID Red dye highlights mitochondria regardless of their energetic state. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as, High Content Screening (HCS) platforms. The kit is useful for assessing mitochondrial morphology changes, estimating mitochondrial mass and co-localizing GFP-tagged proteins to the mitochondrial compartment. Wavelength maxima: MITO-ID Red λex 558 nm, λem 690 nm; Hoechst 33342 λex 350 nm, λem 461 nm. Applications: Fluorescence microscopy, Fluorescent detection .
Enzo Life Sciences LYSO-ID Red detection kit (GFP-CERTIFIED) (100tests)
LYSO-ID Red Lysosomal Detection Kit (GFP-CERTIFIED) is an ideal choice for fluorescence co-localization imaging with GFP-tagged proteins, providing spectrally pure red signal. By contrast, many red-emitting lysosomal probes photoconvert to green fluorescent species or display metachromatic artifacts wherein emission occurs both in the red and green regions of the spectrum, leading to spurious results in GFP co-localization experiments [Freundt et al (2007) Cell Res. 17(11): 956- 958; Nadrigny et al (2007) Biophys J. 93(3):969-980]. The LYSO-ID Red Lysosomal Detection Kit (GFP-CERTIFIED)contains a proprietary acidic organelle-selective dye suitable for staining live cells. The dye is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. Applications: Fluorescence microscopy, Fluorescent detection .
Enzo Life Sciences LYSO-ID Green detection kit (2x96wells)
The LYSO-ID Green detection kit contains an acidic organelle-selective dye suitable for live-cell staining. Conventional fluorescent stains for acidic organelles, such as Acridine Orange (ENZ-52405), form metachromatic artifacts that interfere with multicolor imaging applications. LYSO-ID Green dye generates emission profiles that can be multiplexed with other fluorophores. The dye accumulates in acidic compartments, such as endosomes, lysosomes, and secretory vesicles. Low micromolar concentrations of LYSO-ID Green dye are sufficient for staining mammalian cells. This has been validated with the human cervical carcinoma cell line, HeLa, the human T-lymphocyte cell line, Jurkat, and the human bone osteosarcoma epithelial cell line, U2OS. The LYSO-ID Green dye is a new green-emitting, cell-permeable small organic probe molecule that spontaneously localizes to live cell acidic organelles. Applications: Fluorescence microscopy, Fluorescent detection .