Crosslinking, Labeling and Protein Modification
Crosslinking, Labeling and Protein Modification
Enzo Life Sciences CYTAG CGH Labeling kit (100Reactions)
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations. With optimized, proprietary reagents, the Enzo Life Sciences CGH Labeling Kits for oligo arrays produce high quality data using as little as 0.25 µg of genomic DNA, without a need for pre-amplification. Bulk quantity kit, 2 x 1000 reactions, is available upon special request. For the purification of labeled DNA, use PCR & Gel Clean-up columns, Prod No. ENZ-GEN100. Applications: CGH.
Enzo Life Sciences CYTAG TotalCGH Labeling kit (10Reactions)
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations. With optimized, proprietary reagents, Enzo's CYTAG CGH Labeling Kits produce high quality data using as little as 0.25µg of genomic DNA, without a need for pre-amplification. Bulk quantity kit, 2 x 1000 reactions, is available upon special request. Applications: CGH.
Enzo Life Sciences CYTAG TotalCGH Labeling kit (100Reactions)
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations. With optimized, proprietary reagents, Enzo's CYTAG CGH Labeling Kits produce high quality data using as little as 0.25µg of genomic DNA, without a need for pre-amplification. Bulk quantity kit, 2 x 1000 reactions, is available upon special request. Applications: CGH.
Enzo Life Sciences D-myo-Inositol 1,4,5-trisphosphate . hexapotassium salt (1mg). CAS: 103476-24-0
Product of phosphatidylinositol phospholipid hydrolysis. Causes release of calcium from intracellular stores. Alternative name: Ins(1,4,5)P3 . 6K, IP3. Purity: ≥98% (TLC). Appearance: White to off-white powder. Solubility: Soluble in water. Long Term Storage: -20°C.
Enzo Life Sciences 8-(4-Chlorophenylthio)guanosine 3',5'-cyclic Monophosphate . triethylammonium salt (5mg). CAS: 54364-02-2
Selective activator of cGMP-dependent protein kinase Iα, Iβ and type II as well as of cGMP-gated ion channels. Higher activation potential as compared to cGMP; excellent cell membrane permeability and phosphodiesterase stability. Preferred alternative to unsuitable analogs such as dibutyryl-cGMP. Alternative name: 8-pCPT-cGMP . TEA. Purity: ≥99% (HPLC). Appearance: White to off-white powder. Solubility: Soluble in water. Long Term Storage: -20°C.
Enzo Life Sciences AMPIGENE qPCR 1-Step Probe Kit Lo-ROX (200Reactions)
AMPIGENE qPCR 1-Step Probe Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Probe Kits are designed for use on a variety of probes including: Taqman, Molecular Beacons, and Scorpion probes. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Probe Kits come with either Lo-ROX, Hi-ROX, or No-ROX concentration. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.) Applications: PCR.
Enzo Life Sciences Dihydrorhodamine 123 (ultra pure) (10mg). CAS: 109244-58-8
Dihydrorhodamine 123 is a widely utilized probe for detection of intracellular reactive oxygen species (ROS) such as peroxide, hypochlorous acid and peroxynitrite. It is readily oxidized into rhodamine 123, which exhibits a spectral profile similar to that of FITC. In combination with other fluorescent probes (such as surface receptor-targeted fluorescent antibodies, the cell viability probe propidium iodide, or fluorescent calcium indicators) dihydrorhodamine 123 can be used for multiparametric cell measurements. Wavelength Maxima: Excitation 507nm, Emission 529nm Alternative name: 2-(3,6-Diamino-9H-xanthene-9-yl)-benzoic acid methyl ester. Purity: ≥95% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Enzo Life Sciences Fluorescein-5-maleimide (ultra pure) (25mg). CAS: 75350-46-8
Fluorescein-5-maleimide enables protein labeling via thiol modifications in most proteins at cysteine residues that either are intrinsically present or resulted from reduction of cystine residues. Unlike iodoacetamides, maleimides do not react with histidine and methionine under physiological conditions. The probe is suitable for assessing conformation- dependent accessibility of cysteine residues in proteins. Wavelength Maxima: Excitation 493nm, Emission 515nm Purity: ≥95% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Enzo Life Sciences Chemiluminescent labeling kit (1x5Reactions)
Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and peptides containing primary amine groups. These chemiluminescent molecules are frequently for use as detection reagents in immunoassays as a safe alternative to radioactive tracers. The Chemiluminescent labeling kit provides the necessary components for quick, efficient, and easy acridinium labeling. Applications: Chemiluminescence. Contents: Acridinium NHS Ester, Dry DMF, Bicarbonate labeling buffer, 10% Lysine, Gel filtration column, Column buffer concentrate, Set of Trigger solutions
Enzo Life Sciences Rhod-2 AM (ultra pure) (1mg). CAS: 129787-64-0
Rhod-2 AM has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, making it suitable for use with argon and krypton laser excitation sources. Rhod-2 signal intensity one of the strongest of all the calcium probes. Rhod 2 is especially suitable probe for intracellular calcium monitoring by confocal laser-scanning microscopy and flow cytometry. The dissociation constant of Rhod 2 with calcium is among the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Wavelength Maxima: Excitation 549nm, Emission 578nm. Alternative name: 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N',N'-tetraacetic acid, tetraacetoxy methyl ester . Br. Purity: ≥90% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Enzo Life Sciences AMPIGENE qPCR 1-Step Probe Kit Lo-ROX (1000Reactions)
AMPIGENE qPCR 1-Step Probe Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Probe Kits are designed for use on a variety of probes including: Taqman, Molecular Beacons, and Scorpion probes. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Probe Kits come with either Lo-ROX, Hi-ROX, or No-ROX concentration. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.) Applications: PCR.
Enzo Life Sciences (6) ROX-12-ddUTP (25µl)
(6) ROX-12-ddUTP can be used for 3'-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with (6) ROX-12-ddUTP can be detected by direct fluorescence detection (red fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies. Alternative name: (6) ROX-12-2’,3’- dideoxyuridine-5’-triphosphate. Concentration: 0.1mM. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water. Long Term Storage: -20°C. Excitation maximum: 580 nm. Emission maximum: 603 nm.
Enzo Life Sciences EQ0 NHS ester (1mg)
EQ0 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It can be used to quench reporter dyes: AMCA, AMCA-X, DEAC, DEAC-X, MCA, MCC, CF™ 350, CF™405S, CF™405M, Pacific Blue™, Cascade Blue, Marina Blue, Alexa Fluor 350, Alexa Fluor 405, DyLight 350, DyLight 405, ATTO 390, ATTO 425, ATTO 465, iFluor™350, iFLuor™405, BD Horizon V450, BUV395, eFluor450. Purity: ≥80% (HPLC). Solubility: Soluble in DMSO or DMF. Long Term Storage: -20°C. Applications: Fluorescent detection FRET.
Enzo Life Sciences AMPIGENE qPCR 1-Step Green Kit Lo-ROX (1000Reactions)
AMPIGENE qPCR 1-Step Green Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Green Kits use a proprietary intercalating dye that does not inhibit PCR. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Green Kits come with either Lo-ROX or Hi-ROX concentration. For Hi-ROX, see ENZ-NUC109. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.) Applications: PCR.
Enzo Life Sciences AMPIGENE qPCR 1-Step Green Kit Lo-ROX (200Reactions)
AMPIGENE qPCR 1-Step Green Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Green Kits use a proprietary intercalating dye that does not inhibit PCR. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Green Kits come with either Lo-ROX or Hi-ROX concentration. For Hi-ROX, see ENZ-NUC109. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.) Applications: PCR.