Real Time PCR Master Mixes
Real Time PCR Master Mixes
New England Biolabs, Inc. NEBNext Q5 Hot Start HiFi PCR Master Mix – 50 reactions
NEBNext Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation. However, the NEBNext Ultra II Q5 Master Mix is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
New England Biolabs, Inc. LunaScript RT SuperMix - 500 rxns
LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers allowing even coverage across the length of the RNA targets. In addition LunaScript RT SuperMix contains dNTPs and a blue tracking dye providing a visual indicator that can be followed throughout the two-step RT-qPCR process. This product offers robust linear and sensitive detection using total RNA inputs as high as 1 ug and as low as single copies of RNA.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 2500 rxn (1 x 25 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 500 rxn (5 x 1 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
New England Biolabs, Inc. LunaScript RT SuperMix Kit – 25 reactions
LunaScript RT SuperMix Kit features an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It includes the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, the LunaScript RT SuperMix Kit contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The LunaScript RT SuperMix offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.
New England Biolabs, Inc. WarmStart Colorimetric LAMP 2X Master Mix with UDG – 100 reactions
WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.
New England Biolabs, Inc. Taq 2X Master Mix – 500 reactions (50 µl vol)
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
New England Biolabs, Inc. Quick-Load Taq 2X Master Mix – 500 reactions (50 µl vol)
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. Quick-Load Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl, tracking dyes and stabilizers. The presence of two commonly used tracking dyes for DNA gels, Orange G and Xylene Cyanol FF, gives the master mix a green color, allowing direct loading of the PCR product onto agarose gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at approximately 4 kb and Orange G migrates at approximately 50 bp. The amount of tracking dyes included does not mask co-migrating DNA bands. Quick-Load Taq 2X Master Mix is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
New England Biolabs, Inc. AhdI – 5000 units
A restriction endonuclease that recognizes the sequence GACNN_N^NNGTC.
New England Biolabs, Inc. WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)- 100 rxn
The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is designed to provide a simple one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This mix contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution. The inclusion of thermolabile UDG prevents carryover contamination where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is compatible with multiple detection methods including turbidity detection real-time fluorescence detection and end-point visualization.
New England Biolabs, Inc. LunaScript RT Master Mix Kit (Primer-free) - 100 rxn
LunaScript RT Master Mix Kit (Primer-free) is an optimized master mix containing all the necessary components for first strand cDNA synthesis except for primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers enabling maximum cDNA synthesis flexibility. LunaScript RT Master Mix (Primer-free) features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. In addition, the presence of a blue tracking dye provides a visual indicator for the RT step as well as downstream applications.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG - 2000 rxns
The Luna Probe One-Step RT-qPCR 4X Mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG - 200 rxns
The Luna Probe One-Step RT-qPCR 4X Mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.
New England Biolabs, Inc. WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) – 100 reactions (25 µl vol)
The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 500 rxn (5 x 1 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
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