Gel Electrophoresis Reagents and Buffers
Gel Electrophoresis Reagents and Buffers
New England Biolabs, Inc. NEBuffer™ 3 – 5 ml
New England Biolabs provides a color-coded 10X NEBuffer (with BSA) with each restriction endonuclease to ensure optimal (100%) activity. New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1 , NEBuffer 3.1 and CutSmart Buffer (NEB #B7204) are available.
New England Biolabs, Inc. LAMP Fluorescent Dye - 250 ul
The LAMP fluorescent dye is an intercalating dye that can be used to track a LAMP reaction in real-time by fluorescence measurement. It is provided at 50X concentration and is sufficient for at least 500 LAMP reactions (0.5 ul dye per 25 ul LAMP reaction). The SYBR/FAM channel of common real-time fluorimeters can be used to read the dye.
New England Biolabs, Inc. NEBNext Adaptor Dilution Buffer – 9.6 ml
The NEBNext Adaptor Dilution Buffer is optimized for dilution of the NEBNext Adaptor to the appropriate concentration range for efficient ligation to end-repaired, dA-tailed DNA.
New England Biolabs, Inc. TriDye™ Ultra Low Range DNA Ladder – 1.25 ml
TriDye Ultra Low Range DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing three tracking dyes which serve as visual aids to monitor the progress of migration during native agarose and polyacrylamide gel electrophoresis. The DNA ladder contains 13 bands ranging from 10 bp to 700 bp, most of which are generated from the digestion of a proprietary plasmid with restriction enzymes. The 50 bp and 300 bp bands have increased intensity to serve as reference bands. The ladder is suitable for use with for use with both native polyacrylamide and agarose gel electrophoresis.
New England Biolabs, Inc. Diluent C with rAlbumin - 5 ml
Diluent C (with rAlbumin) is recommended for making dilutions of some NEB restriction enzymes. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Restriction enzymes should be stored at their selling concentrations. Each vial contains 1.3ml of diluent buffer.
New England Biolabs, Inc. OneTaq 2X Master Mix with Standard Buffer – 500 reactions (50 µl vol)
OneTaq 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent DNA Polymerases ideally suited to routine PCR applications from a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The convenient master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
New England Biolabs, Inc. Gel Loading Dye Purple (6X) – 4 ml
Gel Loading Dye, Purple (6X) is the premier gel loading dye from NEB for sharp, tight bands. It is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes. Attention SYBR Safe and GelRed dye users: Due to an increased concentration of SDS in B7024S, NEB recommends using Gel Loading Dye, Purple, No SDS (6X) (NEB# B7025S) instead.
New England Biolabs, Inc. NEBNext dA-Tailing Reaction Buffer – 2 ml
A 10X reaction buffer for use with Klenow Fragment (3´-5´ exo–) in standard NEBNext protocols.
New England Biolabs, Inc. MidRange PFG Marker – 0.05 ml
MidRange PFG Marker consists of concatemers of DNA isolated from the bacteriophage (cI857 ind1 Sam7) mixed with XhoI digested DNA embedded in 1% LMP agarose and supplied in a GelSyringe dispenser. XhoI produces fragments of 15.0 and 33.5 kb. These fragments anneal to and form concatemers with intact DNA. It is designed for use as a size marker for pulsed field gel electrophoresis (PFG). Size range: 15-291 kb.
New England Biolabs, Inc. Quick-Load Purple 50 bp DNA Ladder – 1.25 ml
Quick-load Purple 50 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light. The Quick-Load Purple 50 bp DNA Ladder consists of a proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 50 to 1,350 base pairs. The 200 and 500 bp bands have increased intensity to serve as reference points.
New England Biolabs, Inc. Quick-Load Purple 100 bp DNA Ladder – 3.75 ml
Quick-Load Purple 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing one tracking dye. Tracking dye does not leave a shadow under UV light. Quick-Load Purple 100 bp DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 1,517 bp. The 500 and 1,000 bp bands have increased intensity to serve as reference bands.
New England Biolabs, Inc. Quick-Load 1 kb Plus DNA Ladder – 1.25 ml
Quick-Load 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands. NEB also offers a Quick-Load version of this ladder with purple dye.
New England Biolabs, Inc. Low Range ssRNA Ladder – 100 gel lanes
The Low Range ssRNA Ladder is a set of 6 RNA molecules produced by in vitro transcription of a mixture of 6 linear DNA templates. The ladder sizes are: 1000, 500, 300, 150, 80 and 50 bases. The 300 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing polyacrylamide-urea gels, and on denaturing or native agarose gels.
New England Biolabs, Inc. Quick-Load Purple 1 kb DNA Ladder – 1.25 ml
Quick-Load Purple 1 kb DNA Ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing one tracking dye. Tracking dye does not leave a shadow under UV light. Quick-Load Purple 1 kb DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 10 kb. The 3 kb fragment has increased intensity to serve as reference band.
New England Biolabs, Inc. NEBNext dsDNA Fragmentase Reaction Buffer v2 – 6 ml
The NEBNext dDNA Fragmentase v2 Buffer is an updated version optimized for NEBNext dsDNA Fragmentase performance.