Biochemical Reagents
Biochemical Reagents
Enzo Life Sciences ROS-ID Superoxide detection kit (1Kit)
Enzo Life Sciences’ ROS-ID Superoxide detection kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with a standard orange (e.g., 550/620 nm) fluorescent cube, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Enzo Life Sciences Noladin ether (5mg). CAS: 222723-55-9
Endogenous cannabinoid agonist isolated from porcine brain. Binds specifically to CB1 (Ki=21.2nM) and CB2 (Ki=>3µM) receptors. Causes sedation hypothermia, intestinal immobility and mild antinociception in mice. Chemically stable, but less potent than 2-arachidonoylglycerol (BML-FA028) with an endogenous half-life time of hours rather than minutes. Alternative name: 2-Arachidonyl glyceryl ether, 2-AG ether, 5Z,8Z,11Z,14Z-Eicosatetraen-2-glyceryl ether. Purity: ≥98% (TLC). Solubility: Soluble in DMSO (40mg/ml) or ethanol (25mg/ml). Long Term Storage: -20°C.
Enzo Life Sciences L-Cycloserine (100mg). CAS: 339-72-0
Ketosphinganine synthase inhibitor. Excitatory amino acid. Inhibitor of ketosphinganine synthase (serine-palmitoyl-CoA transferase, EC 2.3.1.50), leading to blockade of sphingosine biosynthesis. Partial agonist at the glycine modulatory site of the NMDA receptor. Alternative name: S(-)-4-Amino-3-isoxazolidinone. Formula: C3H6N2O2. MW: 102.1. MI: 14: 2751. Purity: ≥98% (HPLC). Appearance: White to off-white powder. Solubility: Soluble in water. Use/Stability: Unstable in neutral or acidic solutions. Long Term Storage: -20°C. Handling: Hygroscopic.
Enzo Life Sciences Autocamtide-2-related inhibitory peptide (myristoylated) (0.5mg)
Cell permeable CaM kinase II inhibitor. Corresponds to ALX-151-029 but this one is N-terminal myristoylated to increase cell permeability. Alternative name: AIP (myristoylated). Formula: C78H142N22O20. MW: 1708.1. Purity: ≥98%. Appearance: Lyophilized. Solubility: Soluble in water. Long Term Storage: -20°C.
Enzo Life Sciences HSP90 (human), (native) (200µg)
Alternative name: HSP86, Heat shock protein 90. Purity: ≥90% (SDS-PAGE; Western blot). MW: ~90kDa. Formulation: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 1.0mM DTT and 100mM sodium chloride. Source: Produced in HeLa cells. Long Term Storage: -80°C. Applications: ELISA, WB Antigen microarray.
Enzo Life Sciences beta-Amyloid (1-40) (0.5mg). CAS: 131438-79-4
Formula: C194H295N53O58S. MW: 4329.9. Purity: ≥95%. Appearance: White to off-white powder. Solubility: Soluble in 5% acetic acid. Long Term Storage: -20°C.
Enzo Life Sciences L-Glutathione (oxidized form) (5g). CAS: 27025-41-8
Alternative name: GSSG, (gamma-Glu-Cys-Gly)2. Formula: C20H32N6O12S2. MW: 612.6. Purity: ≥95.0% (HPLC). Formulation: Free acid. Solubility: Soluble in water. Long Term Storage: +4°C.
Enzo Life Sciences Ghrelin (human) (0.5mg). CAS: 258279-04-8
Ghrelin, a 28 aa peptide predominantly produced by the stomach, is an endogenous ligand for the growth hormone secretagogue receptor (GHS-R) that potently stimulates growth hormone release. Studies suggest that peripherally or centrally administrated ghrelin, independent of growth hormone, decreases fat oxidation and increases food intake and adiposity. In addition, plasma ghrelin levels are lower in obese human subjects. Ghrelin promotes the production of orexigenic neuropeptides (neuropeptide Y and agouti-related protein) in the hypothalamic arcuate nuclei and activates the neurons which produce these orexigenic neuropeptides, resulting in an increase in feeding and body weight and thereby opposing the effects of leptin. Moreover, ghrelin shows regulatory effects on the cardiovascular and immune system. Formula: C149H249N47O42. MW: 3370.9. Purity: ≥98%. Formulation: Lyophilized. Solubility: Soluble in distilled water or 5% acetic acid. Long Term Storage: -20°C.
Enzo Life Sciences Melittin (natural) (5mg). CAS: 20449-79-0
Causes disruption of normal cellular activity and cell lysis. Binds calmodulin in a calcium-dependent manner. Activates phospholipase A2 (PLA2) and inhibits protein kinase C (PKC) by binding to the catalytic domain in a Mg-ATP sensitive manner. Antirheumatic. Formula: C131H229N39O31. MW: 2846.5. Purity: ≥85% (HPLC). Appearance: White to faint tan powder. MI: 14: 5824. Solubility: Soluble in PBS (5mg/ml) or water (5mg/ml). Long Term Storage: -20°C. Handling: Very hygroscopic. Protect from light.
Enzo Life Sciences Guanylin (human) (0.1mg). CAS: 183200-12-6
Guanylyl cyclase activator. Endogenous intestinal guanylyl cyclase activator. Formula: C58H87N15O21S4. MW: 1458.7. Purity: ≥96%. Appearance: White lyophilized powder. Solubility: Soluble in water. Long Term Storage: -20°C.
Enzo Life Sciences Ghrelin (human) (1mg). CAS: 258279-04-8
Endogenous GHS-R ligand. Ghrelin, a 28 aa peptide predominantly produced by the stomach, is an endogenous ligand for the growth hormone secretagogue receptor (GHS-R) that potently stimulates growth hormone release. Studies suggest that peripherally or centrally administrated ghrelin, independent of growth hormone, decreases fat oxidation and increases food intake and adiposity. In addition, plasma ghrelin levels are lower in obese human subjects. Ghrelin promotes the production of orexigenic neuropeptides (neuropeptide Y and agouti-related protein) in the hypothalamic arcuate nuclei and activates the neurons which produce these orexigenic neuropeptides, resulting in an increase in feeding and body weight and thereby opposing the effects of leptin. Moreover, ghrelin shows regulatory effects on the cardiovascular and immune system. Formula: C149H249N47O42. MW: 3370.9. Purity: ≥98%. Formulation: Lyophilized. Solubility: Soluble in distilled water or 5% acetic acid.
Enzo Life Sciences Z-Asp-2,6-dichlorobenzoyloxymethylketone (50mg). CAS: 153088-73-4
Irreversible, cell permeable broad-spectrum caspase inhibitor. Caspase-3 IC50: 1,1µM Caspase-6 IC50: 4,1µM Less effective on the inhibition of caspase-1- and 8-like activities. Inhibits hypoxia-induced apoptosis in neuroblastoma cells. Alternative name: Caspase family inhibitor, Z-Asp-CH2-DCB, Z-L-Asp-a-[2,6-(dichlorobenzoyl)oxy]methane. Formula: C20H17Cl2NO7. MW: 454.3. Purity: ≥98% (TLC). Appearance: White to off-white powder. Solubility: Soluble in methanol (50mg/ml) or DMSO. Long Term Storage: -20°C. Use/Stability: Stock solutions should be divided into aliquots and stored at -20°C. Handling: Keep cool and dry.
Enzo Life Sciences Z-DEVD-pNA (1mg)
Chromogenic substrate for caspase-3 with increased cell permeability. Also substrate for caspase-1, -4 and -7. λmax of pNA is 400nm. Similar to Ac-DEVD-pNA except the N-terminus is blocked by a benzyloxycarbonyl (Z) moiety which may increase cell permeability. Alternative name: Caspase 3 substrate (chromogenic). Formula: C32H38N6O14. MW: 730.7. Purity: ≥99% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO or acetonitrile. Long Term Storage: -20°C.
Enzo Life Sciences Ac-IETD-pNA (1mg)
Caspase and granzyme substrate. Chromogenic substrate for caspase-6, -8 and granzyme B. Also a substrate for caspase-10. Similar to Ac-IETD-AMC and Ac-IETD-AFC but cleavage is monitored colorimetrically by absorbance at 405 nm. Alternative name: Caspase-8 substrate (chromogenic), Granzyme B substrate (chromogenic). Formula: C27H38N6O12. MW: 638.6. Purity: ≥98% (HPLC). Formulation: Lyophilized. Peptide content: 70-90%. Solubility: Soluble in DMSO. Dilute with buffer, pH 7.0-7.5. Long Term Storage: -20°C. Handling: Keep cool and dry.
Enzo Life Sciences Z-YVAD-pNA (1mg)
Chromogenic substrate for caspase-1 with increased cell permeability. λmax of pNA is 400nm. Alternative name: Caspase 1 substrate (chromogenic). Formula: C35H40N6O11. MW: 720.7. Purity: ≥97% (HPLC). Appearance: White powder. Formulation: Lyophilized. TFA salt. Solubility: Soluble in DMSO. Can be diluted with basic buffers. Long Term Storage: -20°C. Handling: Keep cool and dry.