DNA Sequencing
DNA Sequencing
New England Biolabs, Inc. NEBNext Enzymatic Methyl-seq Kit – 24 reactions
NEBNext Enzymatic Methyl-seq (EM-seq) is a new method for identification of 5-mC and 5-hmC. While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. In contrast, the highly effective enzyme-based conversion in the NEBNext Enzymatic Methyl-seq Kit minimizes damage to DNA and, with the supplied NEBNext Ultra II library preparation workflow reagents, produces high quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.
New England Biolabs, Inc. NEBuilder HiFi DNA Assembly Master Mix – 250 reactions
NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. It allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Used to assemble either ss oligonucleotides or different sizes of DNA frags with varied overlaps. Has utility in synbio community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple MM format. The rxn includes different enzymes that work together in the same buffer: The exonuclease creates single-stranded 3' overhangs that facilitate the annealing of fragments that share complementarity at one end (the overlap region): a polymerase fills in gaps within each annealed fragment; DNA ligase seals nicks in the assembled DNA. End result is a ds fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation of E coli.
New England Biolabs, Inc. NEBNext Microbiome DNA Enrichment Kit – 24 reactions
The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment.
New England Biolabs, Inc. NEBNext Ultra™ II Ligation Module – 96 reactions
This module is part of the Ultra II workflow, and is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module, for Illumina-compatible library construction. The NEBNext Ultra II Ligation Module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module or the NEBNext Ultra II FS DNA Module. This module is also compatible with some Oxford Nanopore Technologies workflows.
New England Biolabs, Inc. NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) – 96 reactions
The novel NEBNext Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext Small RNA Library Prep Set for Illumina (Set 1) includes adaptors and multiplex primers with 12 indices, to enable multiplexing.
New England Biolabs, Inc. NEBNext Immune Sequencing Kit (Mouse) - 24 rxn
The NEBNext Immune Sequencing Kit (Mouse) enables exhaustive profiling of somatic mutations in the full-length immune gene repertoires of B cells and T cells, via the expression of complete antibody chains. This includes modular primer sets, providing information for complete V, D, and J segments and full isotype information analysis (IgM, IgD, IgG, IgA and IgE), and TCR(alpha symbol),TCR(beta symbol) TCRγ and TCRδ chain characterization. A unique, UMI-based mRNA barcoding process allows PCR copies derived from an individual molecule to be converted to a consensus sequence. This improves sequence accuracy and eliminates PCR bias, allowing for quantitative digital molecule counting. Immune repertoire sequencing is frequently used to analyze immune responses, both current and distant. Areas of particular interest include characterization of autoimmune diseases, oncology, and the discovery of neutralizing antibodies against infectious disease.
New England Biolabs, Inc. NEBNext Immune Sequencing Kit (Human) - 24 rxn
The NEBNext Immune Sequencing Kit (Human) enables exhaustive profiling of somatic mutations in the full-length immune gene repertoires of B cells and T cells, via the expression of complete antibody chains. This includes modular primer sets, providing information for complete V, D, and J segments and full isotype information analysis (IgM, IgD, IgG, IgA and IgE), and TCR(alpha symbol) and TCR(beta symbol) chain characterization. A unique, UMI-based mRNA barcoding process allows PCR copies derived from an individual molecule to be converted to a consensus sequence. This improves sequence accuracy and eliminates PCR bias, allowing for quantitative digital molecule counting. Immune repertoire sequencing is frequently used to analyze immune responses, both current and distant. Areas of particular interest include characterization of autoimmune diseases, oncology, and the discovery of neutralizing antibodies against infectious disease.
New England Biolabs, Inc. NEBNext Ultra™ II End Repair/dA-Tailing Module – 24 reactions
This module is part of the Ultra II workflow, and is optimized for use with the NEBNext Ultra II Ligation Module , for Illumina-compatible library construction. This module is also compatible with some Oxford Nanopore MinIONworkflows.
New England Biolabs, Inc. NEBNext Ultra™ II Ligation Module – 24 reactions
This module is part of the Ultra II workflow, and is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module, for Illumina-compatible library construction. The NEBNext Ultra II Ligation Module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module or the NEBNext Ultra II FS DNA Module. This module is also compatible with some Oxford Nanopore Technologies workflows.
New England Biolabs, Inc. NEBNext UltraExpress RNA 96
The NEBNext UltraExpress RNA Library Prep Kit is the latest generation of NEBNext RNA library prep, and has a fast, streamlined workflow. With a 3-hour library prep protocol, the kit enables creation of high-quality RNA libraries in a single day, in conjunction with mRNA enrichment or rRNA depletion kits.
New England Biolabs, Inc. NEBNext Ultra™ DNA Library Prep Kit for Illumina – 96 reactions
This original version of the Ultra DNA Library Prep Kit enables library preparation from 5 ng – 1 µg of input DNA in a streamlined workflow. However, we now recommend use of the more recent NEBNext Ultra II DNA Library Prep Kit for Illumina, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 µg).
New England Biolabs, Inc. NEBNext Ultra™ II Q5 Master Mix – 250 reactions
The NEBNext Ultra II Q5 Master Mix is the most recent formulation of Q5 DNA polymerase that has been optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries. This new formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´- 5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has the highest fidelity available (> 280-fold higher than that of Taq DNA Polymerase), resulting in ultra-low error rates. The NEBNext Ultra II Q5 Master Mix is an aptamer-based hot start formulation that allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification.
New England Biolabs, Inc. NEBNext Companion Module for Oxford Nanopore TechnologiesLigation Sequencing
The NEBNext DNA repair end repair and ligation reagents recommended in Ligation library preparation are now available in the same product at volumes designed for use in several protocols. The appropriate device-specific protocol from Oxford Nanopore Technologies should be followed for use of these reagents.
New England Biolabs, Inc. NEBNext Immune Sequencing Kit (Mouse) - 96 rxn
The NEBNext Immune Sequencing Kit (Mouse) enables exhaustive profiling of somatic mutations in the full-length immune gene repertoires of B cells and T cells, via the expression of complete antibody chains. This includes modular primer sets, providing information for complete V, D, and J segments and full isotype information analysis (IgM, IgD, IgG, IgA and IgE), and TCR(alpha symbol),TCR(beta symbol) TCRγ and TCRδ chain characterization. A unique, UMI-based mRNA barcoding process allows PCR copies derived from an individual molecule to be converted to a consensus sequence. This improves sequence accuracy and eliminates PCR bias, allowing for quantitative digital molecule counting. Immune repertoire sequencing is frequently used to analyze immune responses, both current and distant. Areas of particular interest include characterization of autoimmune diseases, oncology, and the discovery of neutralizing antibodies against infectious disease.
New England Biolabs, Inc. CpG Methyltransferase (M.SssI) – 500 units
The CpG Methyltransferase, M.SssI, methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5'...CG...3'
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