Antibodies
Antibodies
STEMCELL Technologies Anti-Rat NGF Receptor/p75NTR (CD271) Antibody, Clone 192-IgG, Size: 100 µg
Clone 192-IgG (MC192) is a mouse monoclonal antibody that reacts with CD271 (p75NTR), a 75 kDa type I transmembrane protein. The p75 neurotrophin receptor (p75NTR) is also known as the low-affinity NGF receptor (LNGFR) or CD271. Verified for use with immunohistochemistry assays, use this antibody in applications requiring the detection of the extracellular domain of the neurotrophin receptor p75NTR from rat. This antibody is useful for the detection of all cells expressing p75NTR on their surface, such as motor neurons in the spinal cord, cholinergic basal forebrain neurons, activated Schwann cells, olfactory ensheathing glial cells, and numerous cell lines. Visit STEMCELL.com for more information and related products.
STEMCELL Technologies Human Recombinant LIF, ACF, Size: 100 µg
Use leukemia inhibitory factor (LIF) to regulate a variety of developmental functions. LIF is an interleukin 6 class cytokine that regulates proliferation, invasion, and differentiation of trophoblasts following blastocyst attachment. Upon binding of LIF to LIF receptor (LIFR), LIFR associates with gp130 and activates JAK/STAT and MAPK signaling (Suman et al. JAK-STAT, 2014). LIFR activation of STAT3 is essential for maintaining the mouse embryonic stem cell (ESC) phenotype (Niwa et al. Genes Dev, 1998). Human LIF can be used for the maintenance of mouse ESCs (Dahéron et al. Stem Cells, 2004). Human recombinant LIF from STEMCELL is animal component-free and comes lyophilized with ≥ 95% purity; EC50 ≤ 0.2 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: CDF, Cholinergic differentiation factor, D Factor, DIA, Differentiation-inducing factor, Differentiation inhibitory activity, Emfilermin, Hepatocyte-stimulating factor III, Leukemia inhibitory factor, MLPLI.
STEMCELL Technologies Human Recombinant Oncostatin M, Size: 1000 µg
Use oncostatin M (OSM) to modulate differentiation, cell proliferation, hematopoiesis, and inflammation (Tanaka & Miyajima. Rev Physiol Biochem Pharmacol, 2003). OSM, a member of interleukin 6 family of cytokines, bears close resemblance to both leukemia-inhibitory factor and granulocyte colony-stimulating factor in amino acid sequence and their modulation of differentiation in a variety of cell types (Rose & Bruce. Proc Natl Acad Sci USA, 1991). OSM activates the JAK/STAT pathway by signaling through type I receptor (consisting of gp130 and LIF receptor) and type II receptor (consisting of gp130 and OSM receptor) (Auguste et al. J Biol Chem, 1997; Gómez-Lechón. Life Sci, 1999). OSM is primarily produced by activated T cells and monocytes, as well as activated macrophages, neutrophils, mast cells, and dendritic cells. Human recombinant Oncostatin M from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 10 ng/mL; endotoxin levels verified ≤ 0.2 EU/μg protein. Alternative names: OSM.
STEMCELL Technologies Anti-Human CD56 Antibody, Clone HCD56, Size: 100 µg
Clone HCD56 is a mouse monoclonal antibody that reacts with human CD56 (NCAM), a member of the immunoglobulin (Ig) super family containing 5 Ig-like domains and two fibronectin type-3 domains in its extracellular portion. Verified for use with flow cytometry assays, use this antibody in applications requiring the detection of CD56 (NCAM). The ~140 kDa isoform of CD56 is considered the prototypic cell surface marker on human NK cells. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA) and EasySep™ Human CD56 Positive Selection Kit (Catalog #18055); partial blocking may be observed, as well as EasySep™ HLA CD2 Positive Selection Kit (Catalog #18657HLA) and EasySep™ Human NK Cell Enrichment Kit (Catalog #19055). Visit STEMCELL.com for more information and related products.
STEMCELL Technologies Anti-Mouse CD8a Antibody, Clone 53-6.7, APC, Size: 100 µg
Rat monoclonal IgG2a antibody against mouse, toad CD8a, APC-conjugated
STEMCELL Technologies Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin, Size: 500 µg
Rat monoclonal IgG2a antibody against mouse, toad CD8a, biotin-conjugated
STEMCELL Technologies Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5, Size: 100 µg
Rat monoclonal IgG2a antibody against mouse, toad CD8a, PerCP-Cy5.5-conjugated
STEMCELL Technologies Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor 488, Size: 25 µg
Clone OX-7 is a mouse monoclonal IgG1 antibody that reacts with mouse, rat, and guinea pig CD90/CD90.1, a GPI-linked membrane glycoprotein and member of the immunoglobulin superfamily. Verified for use with flow cytometry assays, use this antibody in applications requiring the detection of CD90/CD90.1. The 25 kDa core protein is N-glycosylated at three sites, giving rise to molecules with a range of molecular masses (25 - 37 kDa). The OX-7 antibody has been reported to induce leukocyte activation, glomerular nephritis, apoptosis in glomerular mesangial cells, and vascular permeability. This antibody clone has been verified for purity assessments of cells isolated from compatible mouse strains with EasySep™ kits, including EasySep™ Mouse T Cell Isolation Kit (Catalog #19851). Visit STEMCELL.com for more information and related products.
STEMCELL Technologies Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor 488, Size: 100 µg
Rat monoclonal IgG2b antibody against mouse CD117 (c-Kit), Alexa Fluor 488-conjugated
STEMCELL Technologies Anti-Rat CD90 Antibody, Clone OX7, PE, Size: 50 µg
Clone OX-7 is a mouse monoclonal IgG1 antibody that reacts with mouse, rat, and guinea pig CD90/CD90.1, a GPI-linked membrane glycoprotein and member of the immunoglobulin superfamily. Verified for use with flow cytometry assays, use this antibody in applications requiring the detection of CD90/CD90.1. The 25 kDa core protein is N-glycosylated at three sites, giving rise to molecules with a range of molecular masses (25 - 37 kDa). The OX-7 antibody has been reported to induce leukocyte activation, glomerular nephritis, apoptosis in glomerular mesangial cells, and vascular permeability. This antibody clone has been verified for purity assessments of cells isolated from compatible mouse strains with EasySep™ kits, including EasySep™ Mouse T Cell Isolation Kit (Catalog #19851). Visit STEMCELL.com for more information and related products.
STEMCELL Technologies Anti-Mouse F4/80 Antibody, Clone BM8, APC, Size: 100 µg
Clone BM8 is a rat monoclonal antibody that reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. Verified for use with flow cytometry assays, use this antibody in applications requiring the detection of human and mouse F4/80. F4/80 is reportedly the only macrophage marker suitable for distinguishing destructive from non-destructive inflammatory processes in the pancreas. The protein is thought to play a role in the generation of CD8+ regulatory T cells involved in immune tolerance. This antibody clone has also been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19761) and EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770). Visit STEMCELL.com for more information and related products.
STEMCELL Technologies Anti-Mouse F4/80 Antibody, Clone BM8, FITC, Size: 50 µg
Clone BM8 is a rat monoclonal antibody that reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. Verified for use with flow cytometry assays, use this antibody in applications requiring the detection of human and mouse F4/80. F4/80 is reportedly the only macrophage marker suitable for distinguishing destructive from non-destructive inflammatory processes in the pancreas. The protein is thought to play a role in the generation of CD8+ regulatory T cells involved in immune tolerance. This antibody clone has also been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19761) and EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770). Visit STEMCELL.com for more information and related products.
STEMCELL Technologies Anti-Mouse F4/80 Antibody, Clone BM8, PE, Size: 25 µg
Clone BM8 is a rat monoclonal antibody that reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. Verified for use with flow cytometry assays, use this antibody in applications requiring the detection of human and mouse F4/80. F4/80 is reportedly the only macrophage marker suitable for distinguishing destructive from non-destructive inflammatory processes in the pancreas. The protein is thought to play a role in the generation of CD8+ regulatory T cells involved in immune tolerance. This antibody clone has also been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19761) and EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770). Visit STEMCELL.com for more information and related products.
STEMCELL Technologies Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PerCP-Cy5.5, Size: 25 µg
Rat monoclonal IgG2b antibody against mouse Gr-1 (Ly-6G/Ly-6C), PerCP-Cy5.5-conjugated
STEMCELL Technologies Anti-Mouse CD4 Antibody, Clone RM4-4, Biotin, Size: 100 µg
The RM4-4 antibody reacts with mouse CD4, an ~55 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobin (Ig) superfamily; CD4 contains four extracellular Ig-like domains. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T-helper/inducer cells), and at lower levels on dendritic cells. In the mouse, CD4 is not expressed by monocytes/macrophages. CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the avidity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. Binding of the RM4-4 antibody does not block binding of antibody clones RM4-5 or GK1.5.