PCR Supplies
PCR Supplies
New England Biolabs, Inc. Phusion HF Buffer Pack – 6 ml (4 x 1.5 ml)
New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.
New England Biolabs, Inc. Standard Taq Reaction Buffer Pack – 6 ml (4 x 1.5 ml)
New England Biolabs supplies a unique 10X reaction buffer for use with Taq DNA Polymerase in reaction systems which require a detergent free, low ionic strength buffer.
New England Biolabs, Inc. Q5 High-Fidelity PCR Kit – 50 reactions
Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions or 200 reactions. For added convenience, gel loading dye, and a Quick-Load DNA LAdder are also included in this kit.
New England Biolabs, Inc. NEBNext Q5 Hot Start HiFi PCR Master Mix – 50 reactions
NEBNext Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation. However, the NEBNext Ultra II Q5 Master Mix is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
New England Biolabs, Inc. LunaScript Multiplex One-Step RT-PCR Kit - 50 reactions
The LunaScript Multiplex One-Step RT-PCR Kit offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction. It contains a reaction mix and an enzyme mix featuring Luna WarmStart Reverse Transcriptase and Q5 Hot Start High Fidelity DNA polymerase. The kit is optimized for multiplex target amplification to enable diagnostics pathogen detection and viral genome sequencing.
New England Biolabs, Inc. LunaScript RT SuperMix - 500 rxns
LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers allowing even coverage across the length of the RNA targets. In addition LunaScript RT SuperMix contains dNTPs and a blue tracking dye providing a visual indicator that can be followed throughout the two-step RT-qPCR process. This product offers robust linear and sensitive detection using total RNA inputs as high as 1 ug and as low as single copies of RNA.
New England Biolabs, Inc. Luna Universal One-Step RT-qPCR Kit – 1000 reactions
In the Luna One-Step RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 2500 rxn (1 x 25 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
New England Biolabs, Inc. Luna Universal Probe One-Step RT-qPCR Kit – 1000 reactions
In the Luna Universal One-Step Probe RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 500 rxn (5 x 1 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
New England Biolabs, Inc. LunaScript RT SuperMix Kit – 25 reactions
LunaScript RT SuperMix Kit features an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It includes the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, the LunaScript RT SuperMix Kit contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The LunaScript RT SuperMix offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.
New England Biolabs, Inc. Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit - 96 rxn
The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit is optimized for real-time qualitative detection of SARS-CoV-2 nucleic acid using hydrolysis probes. It features the Luna Probe One-Step RT-qPCR 4X Mix with UDG, an optimized SARS-CoV-2 Primer/Probe mix containing primers and probes specific to two regions of the SARS-CoV-2 virus N-gene, and a positive control template. The probes have been modified to contain different fluorophores (N1, HEX; N2, FAM) to enable simultaneous observation on two different channels of a real-time instrument. To ensure the integrity of the input material and absence of inhibition, an internal control (IC) primer and probe set, designed to amplify the human RNaseP gene, is also provided in the primer mix. The reverse primer of this target has been modified from the CDC design to target an exon/exon boundary to reduce background amplification from possible contaminating genomic DNA. Amplification of the IC is observed in the Cy5 channel.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) 500 reactions
The Luna Probe One-Step RT-qPCR Mix with UDG (No ROX) is supplied at a 4X concentration and enables higher amounts of sample input which is relevant for applications where RNA present in low abundance is of interest such as pathogen detection. The mix consolidates the components for one-step RT-qPCR into a single tube including Luna WarmStart RT Hot Start Taq DNA Polymerase dNTPs and Murine RNase Inhibitor in an optimized buffer. Combining Hot Start Taq DNA Polymerase with a novel WarmStart-activated reverse transcriptase allows for dual control of enzyme activity via reversible aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling providing added security for setting up reactions at room temperature. This formulation contains no reference dye and is compatible with any instrument that does not require ROX (if ROX normalization is needed ROX can be added.)
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) 2000 reactions
The Luna Probe One-Step RT-qPCR Mix with UDG (No ROX) is supplied at a 4X concentration and enables higher amounts of sample input which is relevant for applications where RNA present in low abundance is of interest such as pathogen detection. The mix consolidates the components for one-step RT-qPCR into a single tube including Luna WarmStart RT Hot Start Taq DNA Polymerase dNTPs and Murine RNase Inhibitor in an optimized buffer. Combining Hot Start Taq DNA Polymerase with a novel WarmStart-activated reverse transcriptase allows for dual control of enzyme activity via reversible aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling providing added security for setting up reactions at room temperature. This formulation contains no reference dye and is compatible with any instrument that does not require ROX (if ROX normalization is needed ROX can be added.)
New England Biolabs, Inc. WarmStart Colorimetric LAMP 2X Master Mix with UDG – 100 reactions
WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.