Protein Analysis Reagents
Protein Analysis Reagents
New England Biolabs, Inc. α-Lytic Protease - 100 µg
alpha-Lytic Protease (aLP) cleaves after Threonine (T), Alanine (A), Serine (S) and Valine (V) residues. Its specificity makes it an orthogonal and alternative protease to others commonly used in proteomics applications, including trypsin and chymotrypsin. Peptides generated by aLP are of similar average length to those of Trypsin.
New England Biolabs, Inc. NEBExpress Ni Resin – 25 ml
NEBExpress Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and beta-Mercaptoethanol.
New England Biolabs, Inc. NEBExpress E. coli Lysis Reagent 100 mL
NEBExpress E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins.
New England Biolabs, Inc. Diluent B with rAlbumin - 5 ml
Diluent B (with rAlbumin) is recommended for making dilutions of some NEB restriction enzymes. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Restriction enzymes should be stored at their selling concentrations.
New England Biolabs, Inc. NEBExpress MBP Fusion and Purification System – 1 set
The NEBExpress MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.
New England Biolabs, Inc. NEB 5-alpha Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
NEB 5-alpha Competent E. coli (High Efficiency) is a derivative of the popular DH5. It is T1 phage resistant and endA deficient for high-quality plasmid preparations.
New England Biolabs, Inc. NEBExpress Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
High efficiency chemically competent E. coli cells suitable for non-T7 protein expression.
New England Biolabs, Inc. T7 Express Competent E. coli (High Efficiency) – 6 x 0.2 ml/tube
High efficiency chemically competent E. coli cells suitable for T7 protein expression.
New England Biolabs, Inc. β-N-Acetylglucosaminidase S - 500 units
b-N-Acetylglucosaminidase S is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b-N-Acetylglucosamine residues from oligosaccharides.
New England Biolabs, Inc. Lambda DNA-Mono Cut Mix – 0.1 ml
The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI. The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 µg load) for approximating the mass of DNA in comparably intense samples of similar size. This product comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS and is suitable for PFGE and standard electrophoresis. The size range is 1,503 bp kb to 48,502 bp.
New England Biolabs, Inc. Protein G Magnetic Beads 1 ml
Protein G Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. Specifically, the beads consist of a truncated form of recombinant Protein G that is covalently coupled to a nonporous superparamagnetic particle. The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. Protein G exhibits high affinity for subclasses of IgG from many species including human, rabbit, mouse, rat and sheep.
New England Biolabs, Inc. Protein Deglycosylation Mix II – 20 reactions
The Protein Deglycosylation Mix II contains all of the enzymes, reagents, and controls needed to remove all N-linked and simple O-linked glycans as well as some complex O-linked glycans. This mix contains enzyme sufficient for 20 reactions or the cleavage of as much as 2 mg of glycoprotein. All of the enzymes and reagents included in the Protein Deglycosyation Mix II are Mass Spectrometry compatible. Following the deglycosylation reaction, samples are ready to be prepared for mass spectrometry analysis.
New England Biolabs, Inc. SHuffle T7 Express Competent E.coli – 12 x 0.05 ml/tube
Chemically competent E. coli cells suitable for T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm.
New England Biolabs, Inc. Luna Cell Ready Lysis Module – 100 reactions
Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps. The module coordinates cell lysis, RNA release, and genomic DNA removal simultaneously, resulting in cell lysate ready for direct RT-qPCR analysis with Luna Universal One-Step RT-qPCR kits. Similar to other Luna products, the lysis buffer includes an inert blue tracking dye for visual assistance throughout the workflow.
New England Biolabs, Inc. Remove-iT PNGase F – 33750 units
Remove-iT PNGase F is a tagged version of PNGase F, optimized for easy removal from a reaction. Remove-iT PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins.