Protein Analysis Reagents
Protein Analysis Reagents
Enzo Life Sciences CYTAG CGH Labeling kit (100Reactions)
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations. With optimized, proprietary reagents, the Enzo Life Sciences CGH Labeling Kits for oligo arrays produce high quality data using as little as 0.25 µg of genomic DNA, without a need for pre-amplification. Bulk quantity kit, 2 x 1000 reactions, is available upon special request. For the purification of labeled DNA, use PCR & Gel Clean-up columns, Prod No. ENZ-GEN100. Applications: CGH.
Enzo Life Sciences CYTAG TotalCGH Labeling kit (100Reactions)
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations. With optimized, proprietary reagents, Enzo's CYTAG CGH Labeling Kits produce high quality data using as little as 0.25µg of genomic DNA, without a need for pre-amplification. Bulk quantity kit, 2 x 1000 reactions, is available upon special request. Applications: CGH.
Enzo Life Sciences CYTAG TotalCGH Labeling kit (10Reactions)
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations. With optimized, proprietary reagents, Enzo's CYTAG CGH Labeling Kits produce high quality data using as little as 0.25µg of genomic DNA, without a need for pre-amplification. Bulk quantity kit, 2 x 1000 reactions, is available upon special request. Applications: CGH.
Enzo Life Sciences Tetanus toxoid (100 µg)
Inactivation with formaldehyde leads to non-toxic form of tetanus toxin. Produced from tetanus toxin by inactivation with formaldehyde. The resulting toxoid is essentially non-toxic. Synonyms: Formaldehyde inactivated tetanus toxin. MW: ~150kDa. UniProt ID: P04958. Formulation: Lyophilized.
Enzo Life Sciences L-alpha-Phosphatidylinositol-3,4-P2 (100µg)
Activates Ca2+-insensitive PKC isotypes δ, ε and η. Activates Akt/PKB by direct interaction with the Akt PH domain. Purity: ≥98% (NMR). Solubility: Soluble in chloroform/methanol/water, 50:50:10 (10mg/ml); not water soluble but resuspends, with vigorous vortexing, to ~0.5 mg/ml in water. See references for method of incorporation into mixed lipid vesicles by sonication, and for dissolving in a detergent-containing buffer. Long Term Storage: -20°C.
Enzo Life Sciences Phosphoramidon (5mg). CAS: 119942-99-3
Inhibits neutral endopeptidase 24.11 (NEP, neprilysin; EC 3.4.24.11) (Ki=2nM), a cell surface zinc metalloprotease which cleaves bioactive peptides such as bradykinin, neurotensin, and enkephalin. Also inhibits neprilysin 2 (Ki~2nM). Potent inhibitor of endothelin-converting enzyme (ECE) (IC50=0.68μM). Alternative name: N-(alpha-Rhamnopyranosyloxyhydroxyphosphinyl)-L-leucyl-L-tryptophan. Purity: ≥97% (HPLC). Solubility: Soluble in water, DMSO or methanol. Long Term Storage: -20°C.
Enzo Life Sciences Apstatin (1mg). CAS: 160470-73-5
Apstatin is a potent and selective inhibitor of aminopeptidase P (APP), Ki=2.6 µM for purified rat lung membrane-bound APP. It blocks the APP-mediated degradation of bradykinin. Limits myocardial infarct size alone or with ACE inhibitors. Alternative name: N-[(2S,3R)-3-Amino-2-hydroxy-4-phenylbutanoyl]-L-prolyl-L-prolyl-L-alaninamide. Purity: ≥95% (HPLC). Formulation: Lyophilized. Solubility: Soluble in water (>25 mg/ml) or DMSO (20 mg/ml). Long Term Storage: -20°C.
Enzo Life Sciences Sphingomyelin (100 mg). CAS: 85187-10-6
Contains primarily stearic and nervoic acids. Precursor to ceramide second messengers via the action of sphingomyelinase. Purity: ≥98% (TLC). Solubility: Soluble in chloroform, ethanol or methanol. Long Term Storage: -20°C.
Enzo Life Sciences FTY-720. HCl (25 mg). CAS: 162359-56-0
Novel immune modulator that alters migration and homing of lymphocytes. Acts as a potent agonist of sphingosine 1-phosphate receptors (S1P) after phosphorylation by sphingosine kinase. Also inhibits cytosolic phospholipase A2 independently of sphingosine 1-phosphate receptors. Alternative name: 2-Amino-2-[2-(4-octylphenyl)ethyl]-1,3-propanediol . HCl, Fingolimod . HCl. Purity: ≥98% (HPLC). Solubility: Soluble in DMSO (10mg/ml), 100% ethanol or dimethyl formamide. Long Term Storage: -20°C.
Enzo Life Sciences Paclitaxel (25 mg). CAS: 33069-62-4
Antitumor agent. Stabilizes microtubules and lowers the critical concentration for tubulin polymerization. Reversibly binds to polymerized tubulin. Inhibits protein isoprenylation and induces apoptosis in PC-3 human prostate cancer cells. The checkpoint of mitotic spindle assembly, aberrant activation of cyclin-dependent kinases (CKDs) and the c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK) are involved in Paclitaxel-induced apoptosis. Purity: ≥98% (HPLC). Solubility: Soluble in DMSO or methanol at 10mg/ml. Long Term Storage: -20°C.
Enzo Life Sciences Ansamitocin P-3 (1 mg). CAS: 66547-09-9
Ansamitocin P-3 is a maytansine analog which displays potent cytotoxicity against the human solid tumor cell lines A-549 and HT-29. It shares the same binding site as rhizoxin and phomopsin A on porcine brain tubulin. Maytansine and its analogs cause extensive disassembly of the microtubule and totally prevent tubulin spiralization. Purity: ≥93% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Enzo Life Sciences Vincristine. sulfate (5 mg). CAS: 2068-78-2
Plant alkaloid that arrests the cell cycle in G2/M phase by blocking mitotic spindle formation. Depolymerizes microtubules and blocks binding of tubulin to microtubule proteins. Causes aberrant tubulin polymerization. Induces apoptosis. Triggers Raf-1 activation, phosphorylation of Bcl-2 family proteins and induction of p53 expression. Cancer chemotherapeutic agent. Posttranscriptionally downregulates HIF-1α expression. Alternative name: VCR . sulfate, Leukocristine . sulfate. Purity: ≥98% TLC. Solubility: Soluble in water (25mg/ml) or methanol. Long Term Storage: -20°C.
Enzo Life Sciences PATHO-GENE HPV screening probe (1ml)
The PATHO-GENE HPV screening probe is a mixture of biotin-labeled HPV 6, HPV 11, HPV16, HPV 18, HPV 31, HPV 33, and HPV 51-specific probes in buffered formamide and hybridization enhancers. Long Term Storage: +4°C.
Enzo Life Sciences MITO-ID Membrane potential detection kit (100tests)
Cell-based assays for evaluating the functional status of mitochondria are emerging as useful tools for elucidating the role of mitochondrial activity in drug-induced toxicity, the apoptosis cascade and other cellular and biochemical processes. The loss of the mitochondrial membrane potential (MMP) is often associated with early stages of apoptosis. The collapse of MMP coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. MITO-ID Membrane Potential Detection Kit measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Enzo Life Sciences PROTEOSTAT PDI assay kit (50tests)
The PROTEOSTAT PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT PDI detection dye. Relative to analogous turbidimetric assays of PDI activity, the fluorescence-based assay provides a vastly improved assay signal window, improved lower detection limit, and superior Z’-score (>0.8). Intra-plate and inter-plate CVs using the assay are typically 3-5%. Human recombinant PDI enzyme (EC 5.3.4.1) and the PDI inhibitor, bacitracin, are included in the kit, as well as all reagents necessary for monitoring changes in PDI activity. Alternative name: Protein disulfide isomerase. Applications: Fluorescent detection .