Cell Based Assays
Cell Based Assays
Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 670
A red fluorescent dye that can be used to monitor individual cell divisions for up to 6 generations.
Invitrogen™ eBioscience™ BrdU Staining Buffer Set for Flow Cytometry
Contains the buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis. This set, in combination with BrdU and DNase I steps, allows for detection of BrdU pulse-labeled cells, thereby enabling the examination of proliferating cells.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 660
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 455UV
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Invitrogen™ pHrodo™ Red Phagocytosis Particle Labeling Kit for Flow Cytometry
pHrodo™ Red Phagocytosis Particle Labeling Kit for Flow Cytometry
Invitrogen™ CellVue™ Maroon Cell Labeling Kit
Far-red fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ eBioscience™ Calcein Blue AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Blue.
Invitrogen™ eBioscience™ CyTRAK Orange™
Anthraquinone dye with high affinity for double-stranded DNA. It is a membrane-permeable dye that can label live or fixed/dead cells.
Invitrogen™ Dextran, Alexa Fluor™ 488; 3,000 MW, Anionic
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
Invitrogen™ DiIC12(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)
When applied to cells, the dye diffuses laterally within the plasma membrane
Invitrogen™ H2DCFDA (H2-DCF, DCF)
Chemically reduced form of fluorescein used as an indicator for reactive oxygen species in cells
Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye
Fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol