Cell Based Assays
Cell Based Assays
Invitrogen™ CellVue™ Plum Cell Labeling Kit
Far-red fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ CellVue™ Jade Cell Labeling Kit
Green fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ eBioscience™ Propidium Iodide
Standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double-stranded DNA, but is excluded from cells with intact plasma membranes.
Invitrogen™ eBioscience™ Propidium Iodide Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Propidium Iodide binds to double-stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes.
Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.
Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.
Invitrogen™ eBioscience™ CFSE
Widely used for cell tracking and proliferation studies. It has also been used in CTL assays and cell motility studies. CFSE readily crosses intact cell membranes.
Invitrogen™ CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry
CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry
Invitrogen™ CoroNa™ Green, AM, cell permeant - Special Packaging
A sodium ion indicator that exhibits an increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength
Invitrogen™ CoroNa™ Green, cell impermeant
A sodium ion indicator that exhibits an increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength
Invitrogen™ Dextran, Fluorescein, 3000 MW, Anionic
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
Invitrogen™ Escherichia coli BioParticles™ Opsonizing Reagent
The Escherichia coli BioParticles™ opsonizing agent is derived from highly purified rabbit polyclonal IgG antibodies.
Invitrogen™ Amplex™ Red and Amplex™ UltraRed Reagents
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
Invitrogen™ Alexa Fluor™ 660 Phalloidin
High-affinity F-actin probe conjugated to bright, photostable, near-infrared fluorescent Alexa Fluor 660 dye