Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye

Fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol

Supplier:  Invitrogen™ C10339

Catalog No. C10339

Includes: Each kit contains enough material for 50 to 250 reactions, depending on reaction volume (typically between 0.1 and 0.5mL).

Description

The Click-iT EdU Alexa Fluor 594 Imaging Kit is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor dye in a fast, highly specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol. The most accurate proliferation-detection methods are based on the incorporation and measurement of nucleoside analogues in newly synthesized DNA, with bromodeoxyuridine (BrdU) a commonly used analogue. BrdU-labeled DNA is quantifyied using anti-BrdU antibodies following DNA denaturation by harsh methods (HCl, heat, or enzymes) to expose the BrdU molecules. This step is time consuming and difficult to perform consistently. The harsh treatment can also adversely effect sample integrity and quality, which makes co-staining with other antibodies challenging.

• Simple: works the first time, every time, in less time
• Efficient: no denaturation steps or harsh treatment required
• Content-rich results: better preservation of cell morphology, antigen structure, and DNA integrity
• Consistent: not dependent on variable antibody lots for detection
• Less hazardous

Superior Proliferation Methodology
The Click-iT EdU Alexa Fluor 594 Imaging Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT EdU, mild fixation and detergent permeabilization is sufficient for the small molecule-based Click-iT EdU detection reagent to gain access to the DNA. As a consequence, the Click-iT EdU imaging kit is not only easy to use, but more accurate and compatible with cell cycle analysis and other intracellular or extracellular targets for truly content-rich results.

The Click-iT assay can be used on cells in culture or in vivo following EdU administration by feeding or injection methods. The Click-iTassay can be used with BrdU in dual pulse experiments by using the anti-BrdU (clone MoBu-1) antibody, which does not cross react with EdU. The Click-iTtechnology is compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed cell labeling.

Cell Analysis, Cell Cycle, Cell Proliferation, Cell Viability, Proliferation and Function, Cellular Imaging, DNA Replication and Repair, Immunocytochemistry (ICC), Immunofluorescence (IF)

Order Info

Shipping Condition: Room temperature

Specifications

• Color: Red
• Detection Method: Fluorescence
• For Use With (Equipment): Confocal Microscope, Fluorescence Microscope
• Dye Type: Alexa Fluor™ 594
• Format: Slide(s)
• Product Line: Alexa Fluor™, Click-iT™, Molecular Probes™
• Shipping Condition: Room Temperature

• Content And Storage: Each kit contains enough material for 50 to 250 reactions, depending on reaction volume (typically between 0.1 and 0.5 ml). Store at 2–6°C, dessicate and protect from light.
• For Use With (Application): Proliferation Assay
• Product Type: Cell Proliferation Kit
• Emission: Visible
• Green Features: Less hazardous
• Quantity: 1 kit

For Research Use Only. Not for use in diagnostic procedures.