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Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.
Supplier: Invitrogen™ 65085439
Description
- Molecular weight: 600g/mol
- Peak excitation: 408nm
- Peak emission: 450nm
- Reconstitute in high-quality, freshly opened DMSO
- Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing
- For fluorescent microscopy, detect using the appropriate filter sets
- For flow cytometric analysis, excite with the violet laser line (405nm) and detect using filters for Pacific Blue™/eFluor™ 450 (450/50)
- Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
Specifications
| Violet | |
| Viability Assay | |
| Dye | |
| eBioscience™ |
| Fluorescence | |
| Fluorescence Microscope, Flow Cytometer | |
| 408⁄450 | |
| 1 mg |
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