DNA Cloning and Mapping and Mutagenesis
DNA Cloning and Mapping and Mutagenesis
Invitrogen™ GeneArt™ Seamless Cloning and Assembly Enzyme Mix
Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction
Thermo Scientific™ Rapid DNA Ligation Kit PROMO
Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minuntes at room temperature.
Thermo Scientific™ pUC18 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Thermo Scientific™ pUC57 DNA
Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.
Thermo Scientific™ Lambda DNA (dam–, dcm–)
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Thermo Scientific™ Lambda DNA
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Thermo Scientific™ Rapid DNA Ligation Kit
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Invitrogen™ GeneArt™ pYES1L Vector with Sapphire™ Technology
A ready-to-use, 9.3 Kb linearized BAC/YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System
Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix
GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.
Thermo Scientific™ pUC19 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Invitrogen™ GeneArt™ Type IIs Assembly Kit, Bsa I
Provides seamless cloning and assembly of up to 8 DNA fragments by simultaneous cleavage and ligation in a single reaction
Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Thermo Scientific™ phiX174 RF1 DNA
Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.
Thermo Scientific™ Fast DNA End Repair Kit
Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.
Cytiva Ready-to-Go™ You-Prime First-Strand Beads
Use for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice. Cytiva Life Sciences™ Ready-to-Go™ You-Prime First-Strand Beads are perfect for RT-PCR applications.