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Invitrogen™ GeneArt™ pYES1L Vector with Sapphire™ Technology
SDP

A ready-to-use, 9.3 Kb linearized BAC/YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System

Supplier:  Invitrogen™ A13287

Encompass

Catalog No. A13287


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Description

Description

GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments. This product only includes the linearized GeneArt™ pYES1L Vector with Sapphire Technology™.

Some of the key features and elements of the GeneArt™ pYES1L Vector with Sapphire Technology™ include:
  • TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
  • ARS⁄CEN origin to allow stable maintenance of the construct in yeast
  • F’ for conjugation
  • oriT for maintenance of large constructs in E.coli
  • Spectinomycin resistance for selection in E.coli
  • 6 Convenient and validated restriction sites flanking the cloning region
For Research Use Only. Not for use in diagnostic procedures.
Specifications

Specifications

Spectinomycin
The product contains 3 vials of linear GeneArt pYES1L Vector with Sapphire Technology at a concentration of 50 ng/mL. Vials contain enough plasmid for 10 cloning reactions using the GeneArt High-Order Genetic Assembly System (needs to be purchased separately).

Store the product at -80°C.

The vector is guaranteed stable for 6 months when properly stored.
Cloning
Up to 10 Fragments
ARS⁄CEN, oriT
Dry Ice
pYES1L Linearized
Seamless Cloning
DNA
10 Reactions
GeneArt™
TRP1
110 kb total (vector plus all inserts)
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For Research Use Only. Not for use in diagnostic procedures.