Cell Lines and Cell Cultures
Cell Lines and Cell Cultures
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 20 x 0.05 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
New England Biolabs, Inc. NEB 10-beta Competent E. coli (High Efficiency) – 1 96-well plate, 20uL/well
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
Clontech Labs 3P Stellar™ Competent Cells, 10 x 100 ul
Stellar Competent Cells are an E. coli HST08 strain that provides high transformation efficiency. These cells can be used in a wide variety of applications—from preparation of cDNA and genomic libraries, to construction of longer-length genomic libraries, to sub-cloning, and even methylated DNA cloning. Stellar Competent Cells lack the gene cluster for cutting foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning methylated DNA. The cells can also be used for blue/white screening (i.e., α-complementation) when transformed with vectors containing the lacZα gene. A pUC19 vector is provided as well as SOC Medium. Stellar Competent Cells are recommended for use with Clontech's In-Fusion PCR Cloning Kits. 10 x 100 ul
Zymo Research Corporation Mix & Go! Competent Cells-TG1 (10 x 100 μl)
Mix & Go E. coli cells are made chemically competent by a unique method that completely eliminates the need for heat shock and related procedures. For transformation, DNA can be added directly to Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108-109transformants/g of pUC19 DNA, which make the cells optimal for cloning, sub-cloning, library construction, etc. Premade Mix & Go cells are supplied as a pack of 10 convenient 100 l/tube aliquots or in a 96-well format (12 x 8 - tube strips) of 50 l/tube.
Zymo Research Corporation Mix & Go! Competent Cells - DH5 Alpha, 96 x 50 µl
Mix & Go E. coli cells are made chemically competent by a unique method that completely eliminates the need for heat shock and related procedures. For transformation, DNA can be added directly to Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108-109 transformants/µg of pUC19 DNA , which make the cells optimal for cloning, sub-cloning, library construction, etc. Premade Mix & Go cells are supplied as a pack of 10 convenient 100 µl/tube aliquots or in a 96-well format (12 x 8 - tube strips) of 50 µl/tube.
Research Products International Corp Mix & Go! Competent Cells-Zymo 5α, 10 x 100 ul, 10 tubes
The Mix & Go! Competent Cells - DH5 Alpha are premade E. coli DH5 alpha competent cells for simple and highly efficient DNA transformation. These DH5 alpha competent cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
Simple 20 Second Transformation: No heat shock! Just add DNA and spread on plate.
High Transformation Efficiencies: Achieve 108 - 109 per µg of plasmid DNA.
Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.
Agilent Technologies BL21-CODON PLUS RIPL CELLS
BL21-CodonPlus (DE3)-RIPL Competent Cells, 10 x 0.1mL, These cells contain extra copies of rare E. coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms
BIOSEARCH TECHNOLOGIES INC OverExpress C43(DE3) Chem Comp Cells, 12 Rxns (SOLOs)
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.Lucigens OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).
BIOSEARCH TECHNOLOGIES INC OverExpress C41(DE3) Chem Comp Cells, 12 Rxns (SOLOs)
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Some strains also available in electrocompetent format.Lucigens OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3).