Recombinant Proteins
Recombinant Proteins
New England Biolabs, Inc. RecA – 1000 µg
E. coli RecA is necessary for genetic recombination, reactions involving DNA repair and UV-induced mutagenesis. RecA promotes the autodigestion of the lexA repressor, umuD protein and lambda repressor. Cleavage of LexA derepresses more than 20 genes. In vitro studies indicate that in the presence of ATP, RecA promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. The reaction has three distinct steps: (i) RecA polymerizes on the single-strand DNA, (ii) the nucleoprotein filament binds the duplex DNA and searches for a homologous region, (iii) the strands are exchanged.
New England Biolabs, Inc. α1-3,4,6 Galactosidase - 200 units
a1-3,4,6 Galactosidase is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing a1-3, a1-4 and a1-6-linked galactose residues from oligosaccharides.
New England Biolabs, Inc. Recombinant Albumin, Molecular Biology Grade – 12 mg
Need to avoid BSA or albumin from an animal source? Recombinant Albumin, Molecular Biology Grade, is a non-bovine derived albumin that can serve as an alternative to Bovine Serum Albumin (BSA). Like BSA, it has been shown to prevent adhesion of enzymes to reaction tubes and pipette surfaces. It also stabilizes some proteins during incubation. Choose Recombinant Albumin, when there is a need to avoid BSA. Please inquire about custom formulations with Recombinant Albumin.
New England Biolabs, Inc. Furin – 50 units
Furin is a recombinant, ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site of Arg-X-X-Arg. However, the enzyme prefers the site Arg-X-Lys/Arg-Arg.
New England Biolabs, Inc. BstNI – 15000 units
A restriction endonuclease that recognizes the sequence CC^W_GG.
New England Biolabs, Inc. Furin – 250 units
Furin is a recombinant, ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site of Arg-X-X-Arg. However, the enzyme prefers the site Arg-X-Lys/Arg-Arg.
New England Biolabs, Inc. AciI – 200 units
A restriction endonuclease that recognizes the sequence C^CG_C.
New England Biolabs, Inc. AclI – 1500 units
A restriction endonuclease that recognizes the sequence AA^CG_TT.
New England Biolabs, Inc. SwaI – 10000 units
A restriction endonuclease that recognizes the sequence ATTT_^AAAT.
New England Biolabs, Inc. SrfI – 2500 units
A restriction endonuclease that recognizes the sequence GCCC^GGGC.
New England Biolabs, Inc. T4 Gene 32 Protein – 100 µg
T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein required for bacteriophage T4 replication and repair. It cooperatively binds to and stablizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication. It also has been used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structures. Recently, it has been shown to improve restriction enzyme digestion, improve the yield and efficiency of reverse transcription (RT) reactions during RT-PCR, enhance T4 DNA polymerase activity, as well as increase the yield of PCR products.
New England Biolabs, Inc. NEBExpress GamS Nuclease Inhibitor – 75 µg
NEBExpress GamS Nuclease Inhibitor is a recombinant protein that inhibits Exonuclease V (RecBCD) activity and stabilizes linear DNA templates in E. coli based in vitro protein synthesis reactions.
New England Biolabs, Inc. SNAP-tag Purified Protein – 50 µg
The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT).
New England Biolabs, Inc. ET SSB – 50 µg
ET SSB (Extreme Thermostable Single-Stranded DNA Binding Protein) is a single-stranded DNA binding protein isolated from a hyperthermophilic microorganism, which remains fully active after incubation at 95C for 60 minutes. Due to the extreme thermostability, ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.
New England Biolabs, Inc. RNase B – 250 µg
RNase B is a high mannose glycoprotein that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates.
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