Biochemical Reagents
Biochemical Reagents
Eurofins Discoverx KILR Retroparticles for Adherent Cells (Hygromycin B), 4 vials x 0.5. (97-0005)
The KILR Retroparticles for Adherent Cells (Hygromycin B) are MMLV particles designed to transduce adherent cells. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 4 vials of cryopreserved retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-adherent-cells-(hygrom-97-0005.
Eurofins Discoverx KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B), 2 vials x 0.5 (Adherent), 2 vials x 1.0 (Suspension). (97-0008)
The KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B) are MMLV particles designed to transduce adherent and suspension cells respectively. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 2 vials each of cryopreserved adherent and suspension retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-suspension-adherent-c-97-0008.
Eurofins Discoverx Glucagon, 1 mg. (92-1087)
Glucagon is a peptide hormone secreted by the pancreas to increase blood glucose in response to low glucose levels in the blood. Works in opposition to insulin. May be used medically to combat hypoglycemia, beta blocker overdose, anaphylaxis, and with impacted food bolus to relax the lower esophageal sphincter. Acts as an agonist for GCGR. Provided as 1 mg of dry powder. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx 2-Arachidonylglycerol, 10 mg. (92-1226)
2-Arachidonylglycerol is an endocannabinoid ester formed by arachidonic acid and glycerol that is expressed in the CNS at much higher levels than anandamide. Has neuromodulatory effects, and is a potent agonist of the GPR55, CB1, and CB2 receptors (EC50=3, 519, and 618 nM, respectively). Provided as 10 mg of dry powder. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx Mnk2, active, 10 µg. (14-664)
N-terminal 6xHis-tagged recombinant, human full length Mnk2. For detailed product information, visit www.discoverx.com/proteins.
Eurofins Discoverx Oxytocin, 5 mg. (92-1060)
Oxytocin is a nine amino acid neurohypophysial hormone that acts as a neuromodulator in the brain. It is produced in the hypothalamus and secreted from the pituitary, and controls key parts of the reproductive system and brain, especially active during and after childbirth. Acts as an agonist for OXTR. Provided as 5 mg of dry powder. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
![Eurofins Discoverx [Lys-des-arg9]-Bradykinin, 1 mg. (92-1085)](https://assets.fishersci.com/TFS-Assets/CCG/product-images/92-1085.PNG-250.jpg)
Eurofins Discoverx [Lys-des-arg9]-Bradykinin, 1 mg. (92-1085)
Lys-[Des-Arg9]-Bradykinin is a 9 amino acid inflammatory mediating peptide that causes endothelium-dependent vasodilation and hypotensive effects. ACE inhibitors act by increasing bradykinin levels. Potent and selective bradykinin B1 receptor agonist (Ki=0.12 and >30000 nM for B1 and B2 receptors, respectively). Lys-[Des-Arg9]-bradykinin is 16-fold more potent for B1 than [Des-Arg9]-bradykinin. Provided as 1 mg of dry powder alongside reconstitution buffer. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx Orexin A, 1 mg. (92-1123)
Orexin A is a neuropeptide hormone released by the hypothalamus that excites neurons and affects hunger and wakefulness by modulating the dopamine, norepinephrine, histamine, and acetylcholine systems. Lack of orexin A tends to result in narcolepsy. Potent orexin receptor agonist (Ki=20 and 38 nM for OX1 and OX2, respectively). Provided as 1 mg of dry powder. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
![Eurofins Discoverx [Lys8,9]-Neurotensin (8-13), 5 mg. (92-1059)](https://assets.fishersci.com/TFS-Assets/CCG/product-images/92-1059.PNG-250.jpg)
Eurofins Discoverx [Lys8,9]-Neurotensin (8-13), 5 mg. (92-1059)
[Lys8,9]-Neurotensin (8-13) is the smallest active fragment of Neurotensin, a 13 amino acid neuropeptide of the CNS that is implicated in luteinizing hormone (LH) and dopamine regulation, and prolactin release. Effects of Neurotensin activity include vasodilation, analgesia, hypothermia, and increased locomotion. This [Lys8,9]-analog of neurotensin enhances amiloride-sensitive Na+-dependent transmembrane H+ flux. Provided as 5 mg of dry powder. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx Insulin, 10 mg. (92-1088)
Insulin is a peptide hormone secreted by the pancreas in response to high glucose levels in the blood, leading to decreased blood glucose. It also regulates the metabolism of carbohydrates and fats. Insulin works in opposition to glucagon, and also inhibits the production of glucose by the liver. Patients with metabolic disorders, like type I and type II diabetes mellitus and metabolic syndrome, either do not have a constant proportion of insulin in the blood or are insensitive to its effects. Insulin is commonly used medically to treat some forms of diabetes. Acts as an agonist for INSRa and INSRb. Provided as 10 mg of dry powder. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx Endothelin-1, 10 μg. (92-1164)
Endothelin 1 is an endothelium derived vasoconstrictive peptide produced in vascular endothelial cells. It is one of the three isoforms of endothelin, and a potent agonist of the endothelin receptors type A and type B (EDNRA/ETA and EDNRB/ETB). Widely expressed in all tissues, and responsible for maintenance of vascular tone. Inhibitors and antagonists are used to treat pulmonary hypertension. Provided as 10 μg of dry powder alongside reconstitution buffer. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx L-Glutamic Acid, 10 mg. (92-1197)
L-glutamic acid (glutamic acid) is a naturally produced proteinogenic non-essential amino acid that plays a role in neural activation. It is the most common excitatory neurotransmitter in the CNS. Acts as an agonist of the glutamate receptors (metabotropic, kainate, nMDA, and AMPA). May be dysregulated in nervous system disorders, such as epilepsy. Provided as 10 mg of dry powder. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx Neuromedin B, 1 mg. (92-1233)
Neuromedin B ( nMB) is a mammalian peptide related to bombesin that is present in the CNS and GI tract. It acts as a regulator of exocrine and endocrine secretions, cell growth, body temperature, smooth muscle contraction, and blood pressure and glucose levels. Potent agonist of the neuromedin B receptor NMBR (IC50=2 nM). Provided as 1 mg of dry powder. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx QRFP-26/P518, 100 µg. (92-1194)
QRFP-26 (P518) is a 26 amino acid RF-amide peptide found in the brain that is a potent agonist of the G protein-coupled receptor 103 (GPR103). Causes aldosterone release, and may thus regulate adrenal function. Stimulates feeding behavior, metabolic rate, and blood pressure. Provided as 100 μg of dry powder alongside reconstitution buffer. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.
Eurofins Discoverx Erythropoietin human, 10 µg. (92-1224)
Erythropoietin (EPO) is a glycoprotein renal hormone responsible for promoting erythropoiesis (formation of red blood cells) by bone marrow. Oxygen scarcity or bleeding stimulate release of EPO. It can be used to test for and treat certain blood conditions like anemia, and has been misused by athletes as a performance-enhancer. Acts as an agonist for EpoR. Provided as 10 μg of dry powder alongside reconstitution buffer. These highly purified, pre-validated control ligands have been used to qualify our InCELL Hunter, PathHunter and cAMP Hunter cell lines as well as our Assay Ready Kits. Ligands are conveniently supplied with a recommended reconstitution buffer. For detailed information and product-specific datasheet, visit www.discoverx.com/ligands.