CD25 (IL-2 Receptor α) Mouse, anti-Human, RB780, Clone: 2A3, BD Horizon™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB568689	BD Biosciences 568689	25 Tests
BDB568688	BD Biosciences 568688	100 Tests

Description

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα). CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γ chain or γc) to form the high-affinity signal-transducing IL-2R complex. CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. CD25 can also be expressed by activated B cells and macrophages. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

Specifications

• Antigen: CD25 (IL-2 Receptor α)
• Applications: Flow Cytometry
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3559
• Isotype: IgG1 κ
• Content And Storage: Refrigerate (2°C to 8°C)

• Clone: 2A3
• Conjugate: RB780
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Immunogen: Human Phytohemagglutinin-activated T Cells
• Primary or Secondary: Secondary