CD25 (IL-2 Receptor α) Mouse, anti-Human, RB780, Clone: 2A3, BD Horizon™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 568688

Catalog No. BDB568688

Description

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα). CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γ chain or γc) to form the high-affinity signal-transducing IL-2R complex. CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. CD25 can also be expressed by activated B cells and macrophages. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

Specifications

• Antigen: CD25 (IL-2 Receptor α)
• Clone: 2A3
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Immunogen: Human Phytohemagglutinin-activated T Cells
• Regulatory Status: RUO
• Gene ID (Entrez): 3559
• Content And Storage: Refrigerate (2°C to 8°C)

• Applications: Flow Cytometry
• Conjugate: RB780
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Secondary
• Target Species: Human
• Isotype: IgG1 κ