Flow Cytometry Compensation, Counting and Calibration
Flow Cytometry Compensation, Counting and Calibration
Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #2
Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.
Invitrogen™ 123count eBeads™ Counting Beads
Intended for use in absolute counting of cells or other particles by flow cytometry. 123count eBeads are 7μm microparticles containing encapsulated dyes compatible with blue (488nm) and violet (405nm) excitation sources and emitting fluorescence between approximately 500nm and 750nm.
Invitrogen™ UltraComp eBeads™ Compensation Beads
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
Invitrogen™ eBioscience™ IC Fixation Buffer
Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
Invitrogen™ eBioscience™ Fixation/Permeabilization Concentrate
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines
Invitrogen™ BrightComp eBeads™ Compensation Bead Kits
BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.
Invitrogen™ AccuCheck™ ERF Reference Particles
Used for standardization and calibration of an intensity unit of fluorescence for flow cytometry analysis
Molecular Probes™ CountBright™ Absolute Counting Beads, for flow cytometry
CountBright™ Absolute Counting Beads, for flow cytometry
Invitrogen™ CountBright™ Plus Ready Tubes
CountBright Plus Ready Tubes offer the same advantages as CountBright Plus Absolute Counting Beads, but in ready-to-use tubes. This not only improves the accuracy and repeatability of cell counting, but also allows for convenient room temperature storage for up to 12 months.
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent
For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.
Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #1
Formulated and optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscience™ Mouse Regulatory T Cell Staining Kit #1 includes: Anti-Mouse CD4 FITC, CD25 APC and CD16/32, Anti-Mouse/Rat Foxp3 PE, Rat IgG2a Isotype Control PE, staining buffer, fixation concentrate.
Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)
Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.
Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X)
Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #3
Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.