Flow Cytometry Compensation, Counting and Calibration
Flow Cytometry Compensation, Counting and Calibration
Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #3
Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.
Invitrogen™ eBioscience™ Human Regulatory T Cell Whole Blood Staining Kit
Contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3+ cells in whole blood samples.
Invitrogen™ CountBright™ Plus Absolute Counting Beads
Calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.
Invitrogen™ eBioscience™ Permeabilization Buffer (10X)
Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.
Invitrogen™ Cell Sorting Set-up Beads (for blue lasers)
Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.
Invitrogen™ eBioscience™ Mouse Th17 Cytokine Staining Panel
This mouse Th17 cytokine staining panel includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage: IL-17A, IL-17F, IL-21 and IL-22.
Invitrogen™ AccuCheck™ ERF Reference Particles
Used for standardization and calibration of an intensity unit of fluorescence for flow cytometry analysis
Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X)
Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)
Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent
For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.
Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #1
Formulated and optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscience™ Mouse Regulatory T Cell Staining Kit #1 includes: Anti-Mouse CD4 FITC, CD25 APC and CD16/32, Anti-Mouse/Rat Foxp3 PE, Rat IgG2a Isotype Control PE, staining buffer, fixation concentrate.
Molecular Probes™ CountBright™ Absolute Counting Beads, for flow cytometry
CountBright™ Absolute Counting Beads, for flow cytometry
Invitrogen™ CountBright™ Plus Ready Tubes
CountBright Plus Ready Tubes offer the same advantages as CountBright Plus Absolute Counting Beads, but in ready-to-use tubes. This not only improves the accuracy and repeatability of cell counting, but also allows for convenient room temperature storage for up to 12 months.