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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)

Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.
Supplier: Invitrogen™ 88882388

Alert:
Contains Sodium azide and formaldehyde
Includes:
- Brefeldin A solution
- IC Fixation Buffer
- Permeabilization Buffer (10X) . Permeabilization Buffer is supplied as a 10X concentrate. 1X Permeabilization Buffer contains 0.1% saponin and 0.09% sodium azide.
Description
- Fixation buffer is used to cross-link, stabilize, and fix the cell membrane
- Permeabilization buffer is used to reversibly permeabilize the cell membrane so the staining antibodies can enter the cell effectively
Specifications
Reagent | |
eBioscience™ | |
Fixation Reagent, Permeabilization Reagent |
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For Research Use Only. Not for use in diagnostic procedures.