Protein Assays and Amino Acid Analysis
Protein Assays and Amino Acid Analysis
New England Biolabs, Inc. β-N-Acetylglucosaminidase S - 500 units
b-N-Acetylglucosaminidase S is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b-N-Acetylglucosamine residues from oligosaccharides.
New England Biolabs, Inc. PURExpress In Vitro Protein Synthesis Kit – 10 reactions
A rapid method for gene expression analysis, PURExpress is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURExpress system preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
New England Biolabs, Inc. K.lactis Protein Expression Kit – 1 set
The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis -mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.
New England Biolabs, Inc. p-Nitrophenylphosphate (PNPP) – 5 ml
p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases.
New England Biolabs, Inc. Amylose Resin – 100 ml
Amylose resin is an affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). It is intended for use in a gravity flow column.
New England Biolabs, Inc. Instant Sticky-end Ligase Master Mix – 250 reactions
Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer. Specifically formulated to rapidly ligate cohesive-end (2-4 bp) substrates and improve transformation, the mix simplifies reaction set-up and provides an optimized ratio of enzyme and buffer components. No thawing of the master mix is required as it maintains a liquid state during storage at -20C and no incubation time is necessary to achieve ligation efficiencies sufficient for successful sub-cloning of sticky-end substrates. Just add the master mix to DNA with compatible ends, mix and transform; thereby reducing the valuable time needed for routine ligations. Ligations for subcloning can be carried out in small volumes with low concentrations, allowing users to conserve precious DNA samples, and be used directly to transform many strains of chemically competent E. coli without dilution.
New England Biolabs, Inc. p-Nitrophenylphosphate (PNPP) – 1 ml
p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases.
New England Biolabs, Inc. α1-3,4 Fucosidase - 200 units
1-3,4 Fucosidase, clones from almond meal, and also known as AMF, is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing 1-3 and 1-4 linked fucose residues from oligosaccharides and glycoproteins.
New England Biolabs, Inc. β1-4 Galactosidase S - 400 units
b1-4 Galactosidase S is a highly specific exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b1-4 linked galactose residues from oligosaccharides.
New England Biolabs, Inc. β-N-Acetylglucosaminidase S - 100 units
b-N-Acetylglucosaminidase S is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b-N-Acetylglucosamine residues from oligosaccharides.