Desalting Columns
Desalting Columns
Disposable or reusable gel filtration chromatography columns for use in protein biology or DNA extraction and purification, for the fractional purification of proteins, nucleic acids, and other macromolecules from low molecular-weight buffer salts and reagents.
Desalting columns are special kinds of gel filtration (exclusion) chromatography columns suitable for the fractional purification of proteins, nucleic acids, and other macromolecules. Their use offers a more convenient and quicker alternative to dialysis.
Desalting columns may be disposable or reusable plastic or glass columns prepacked with beads of a suitable media, typically polysaccharide (Sephadex), or porous polyacrylamide that are more resistant to enzymatic degradation. The beads have defined molecular weight cut-off (MWCO) exclusion points (typically around 6000 Da) that trap, retain, and slow down the elution of small molecules below the MWCO, but exclude and therefore do not impede the more rapid elution of larger macromolecules such as proteins.
Desalting columns can achieve high sample recovery yields of >95%, with column capacities ranging from 5 μL to 10 mL that are suitable for applied sample volumes anywhere between 2 μL and 4 mL.
Types of Desalting Columns
• Columns designed for simple gravity flow
• Spin columns designed especially for centrifugation
• Pressure-compatible columns designed for use with suitable pumps or with integrated preparative LC systems
How Are Desalting Columns Used?
• Removal of salts from protein samples
• Buffer exchange prior to downstream electrophoresis or affinity chromatography
• Removal of unreacted radioactive labels
• Removing phenol or free nucleotides from nucleic acid preparations
• Removing of primers or dyes from PCR or sequencing reactions
• Removal of other low molecular weight compounds such as cross-linking, labelling or derivatization reagents, cofactors, inhibitors, or other contaminants
Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL
Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.
Thermo Scientific™ Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL
The new and enhanced Zeba Spin and Micro Spin Desalting columns and plates are designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They provide exceptional desalting and protein recovery performance.
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
Thermo Scientific™ Pierce™ Polyacrylamide Desalting Columns, 1.8K MWCO, 5 mL
Thermo Scientific Pierce 1.8K MWCO (molecular-weight cutoff) Polyacrylamide Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low-molecular-weight buffer salts and reagents.
Cytiva Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Cytiva PD SpinTrap™ G-25 Desalting Columns
Use for convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr). Cytiva Life Sciences™ PD SpinTrap™ G-25 Desalting Columns provide the option of running multiple samples in parallel.
Cytiva HiTrap™ Desalting Prepacked Columns
For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances.
Promotions are available
Thermo Scientific™ Pierce™ Dextran Desalting Columns (5K MWCO)
Ready-to-use, gel-filtration Thermo Scientific Pierce Dextran Desalting Columns can separate proteins and macromolecules from low-molecular weight buffer salts and reagents up to 5000 Daltons (5 kDa) in 5- or 10-mL volumes.
Cytiva Disposable PD-10 Columns
Use for gravity flow purification. Disposable PD-10 Columns are economical, plastic columns that can be packed with a wide range of bulk media.
Cytiva HiPrep™ 26/10 Desalting Columns
Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15mL per column. Cytiva HiPrep 26/10 Desalting Column is a convenient, easy-to-use column prepacked 53mL column with Sephadex G-25 Fine for desalting and buffer exchange.
Cytiva Superdex 75 Increase Prepacked Column, 5/150 GL
Use this column for rapid purity check and screening of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva Superdex 75 Increase Prepacked Columns are ideal when time is limited and low buffer and sample consumption is important.
Cytiva Superdex 75 Increase Prepacked Column, 10/300 GL
Use these columns in preparative purification as well as for characterization and analysis of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva HisTrap™ excel Prepacked Columns feature tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Cytiva Superdex 75 Increase Prepacked Column, 3.2/300
Use this column for rapid purity check and screening of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva Superdex 75 Increase Prepacked Columns are ideal when time is limited and low buffer and sample consumption is important.
Thermo Scientific™ Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL
Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with Thermo Scientific Pierce 7K MWCO (molecular-weight cutoff) Polyacrylamide Spin Desalting Columns, which are porous polyacrylamide-based gel-filtration columns for biological sample preparation.
