Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL

Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Catalog Number	Mfr. No.	Description	Quantity
PIA44296	Thermo Scientific™ A44296	Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL	25 Columns
PIA44297	Thermo Scientific™ A44297	Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL	2 x 25 Columns
PIA44299	Thermo Scientific™ A44299	Zeba™ Dye and Biotin Removal Spin Columns, 2 mL	25 Columns
PIA44300	Thermo Scientific™ A44300	Zeba™ Dye and Biotin Removal Spin Columns, 5 mL	5 Columns
PIA44301	Thermo Scientific™ A44301	Zeba™ Dye and Biotin Removal Spin Columns, 5 mL	25 Columns
PIA44302	Thermo Scientific™ A44302	Zeba™ Dye and Biotin Removal Spin Columns, 10 mL	5 Columns
PIA44303	Thermo Scientific™ A44303	Zeba™ Dye and Biotin Removal Spin Columns, 10 mL	5 x 5 Columns
PIA44304	Thermo Scientific™ A44304	Zeba™ Dye and Biotin Removal Filter Plates, 96-well	2 Plates
FERA44305	Thermo Scientific™ A44305	Zeba™ Dye and Biotin Removal Filter Plates, 96-well	2 x 2 Plates

Description

Quickly remove non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with a 7K MWCO in fewer than 15 minutes with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. Pierce Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. When using the appropriate amount of sample and working within buffer conditions, these columns and filter plates can successfully remove almost any fluorescent dye. Even dilute protein samples (25 μg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). These products are not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns and filter plates include:
• Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
• High recovery—low-binding resin maximizes protein recovery
• Easy-to-use—no cumbersome column preparation or equilibration
• Fast—small-molecule removal and protein recovery in less than 15 minutes
• Flexible—available in spin columns and filter spin plates for a range of needs

Sample capacities
• 0.5-mL spin column: 50 to 120 μL sample
• 2-mL spin column: 400 to 700 μL sample
• 5-mL spin column: 1 to 2 mL sample
• 96-well filter plate: 40 to 100 μL sample (per well)

Available formats
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Specifications

• Shipping Condition: Room Temperature
• Column Type: Proprietary Resin
• Product Line: Zeba™