Competent Cells

Competent Cells
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Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.


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Shipping Condition | Dry Ice |
---|---|
Blue/White Screening | Yes (lacZΔM15) |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | High Copy Plasmid |
Reduces Recombination | Yes (recA1) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Streptomycin) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | No |
Thermo Scientific™ BL21(DE3) Competent Cells
Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

Thermo Scientific™ DH5α Competent Cells
Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).


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Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.


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Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells
MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.


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Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Blue/White Screening | Yes (lacZΔM15) |
Cloning Unstable DNA | No |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 1010 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | May be used for plasmids >20 kb |
Reduces Recombination | Yes (recA13) |
T1 Phage - Resistant (tonA) | Yes (Streptomycin) |
Antibiotic Resistance Bacterial | No |
Format | Tube |
Product Type | Electrocompetent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | No |
Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli
The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format.


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Invitrogen™ One Shot™ BL21(DE3)pLysS Chemically Competent E. coli
One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Blue/White Screening | No |
Cloning Methylated DNA | No |
Transformation Efficiency Level | Medium Efficiency (1 x 108 to 1 x 109 cfu/μg) |
ToxicProteins | No |
High-throughput Compatibility | Low |
Improves Plasmid Quality | No |
ImprovesProteinStability | Yes (lon, ompT) |
Promoter | T7 |
Preparing Unmethylated DNA | Yes (dcm) |
Reduces Recombination | No |
ImprovesRNAStability | Yes (pLysS) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Chloramphenicol) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (B) |
Product Line | One Shot™ |
Contains F' Episome | No |
Gibco™ Expi293™ Met (–) Expression Medium
Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells.


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Invitrogen™ Tali™ Cell Cycle Kit
Convenient and accurate determination of the percentage of cells in each phase of the cell cycle


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Invitrogen™ Library Efficiency™ DH5α Competent Cells
Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MAX Efficiency™ DH5αF'IQ Competent Cells
MAX Efficiency DH5αF'IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits.


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Invitrogen™ ElectroMAX™ DH10B Cells
ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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