Invitrogen™ MAX Efficiency™ DH5αF'IQ Competent Cells

MAX Efficiency DH5αF'IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits.

Supplier:  Invitrogen™ 18288019

Catalog No. 18-288-019

Includes: 3mL of lawn cells and a vial of monomer M13mp19 RF DNA as a control

Description

MAX Efficiency DH5αF´IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits. The DH5αF´IQ strain contains an F´ episome and is a host for M13mp cloning vectors. It also supports cloning plasmids with an f1-like origin of replication (phagemids) or vector that uses expression from the lac promoter. DH5αF´IQ cells can yield >1 x 10⁸ plaque forming units/μg M13mp19 RF with non-saturating amounts (5 pg) of DNA.

MAX Efficiency DH5αF´IQ Competent Cells have the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. They also carry the recA1 mutation which helps to reduce the rate of recombination while propagating plasmid DNA and the endA1 mutation that increases plasmid DNA yield and quantity.

MAX Efficiency DH5αF´IQ Competent Cells offer:
• >3 x 10⁸ transformants/μg efficiency
• >1 × 10⁸ plaque forming units/μg M13mp19 RF
• Stable F´ episome that eliminates need for growth on minimal media
• lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
• Plasmid DNA maintenance with minimum recombination events due recA1 mutation
• endA1 mutation that enables increased plasmid yield and quantity

Note: DH5αF'IQ lawn cells are provided to allow for plaque formation and a vial of monomer M13mp18 RF DNA are supplied as a control.

Genotype
F^– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (r_K–, m_K+) phoA supE44 λ^–thi^–1 gyrA96 relA1/F′ [proAB+lacIqZΔM15 zzf::Tn5(KmR)] 
 
Find the strain and format that fits your needs
• The DH5α strain and its derivatives are available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats.
• DH5α-T1^R competent cells are available in MultiShot format for high throughput applications.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Chemically Competent Cells
• Improves Plasmid Quality: Yes (endA1)
• Transformation Efficiency Level: Medium Efficiency (1 x 10⁸ to 1 x 10⁹ cfu/μg)
• Antibiotic Resistance Bacterial: Yes (Kanamycin)
• Blue/White Screening: Yes (lacZΔM15)
• Preparing Unmethylated DNA: No
• Shipping Condition: Dry Ice
• Species: E. coli (K12)
• Format: Tube
• Quantity: 5 x 200 μL

• Contains F' Episome: Yes
• Cloning Methylated DNA: No
• Content And Storage: • MAX Efficiency DH5αF'IQ Competent Cells (5 x 200 μL)
  • F'IQ Lawn Cell Assy (2 x 1.5 mL)
  • M13mp18 RF DNA (1 vial)
  Store at –80°C.
  
  • S.O.C. Medium (2 x 6 mL)
  Store S.O.C. Medium at 4°C or room temperature.
  
  
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• High-throughput Compatibility: Low
• Reduces Recombination: Yes (recA1)
• T1 Phage - Resistant (tonA): No
• Promoter: Laclq
• Product Line: MAX Efficiency™

For Research Use Only. Not for use in diagnostic procedures.