Transcription and Translation Reagents
Transcription and Translation Reagents
Enzo Life Sciences BIO-PROBE Blur 8 probe (2µg)
The Blur 8 BIO-PROBE labeled probe is prepared by nick translation of a clone of the human alu repeat sequence. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis). Concentration: 20µg/ml. Formulation: Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA. Long Term Storage: -20°C.
Enzo Life Sciences Fluorescein 12 UTP (250 nmol)
Fluorescein-12-UTP is ideal for in vitro transcription reactions catalyzed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridization to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridization procedures. Alternative Name: Fluorescein-12-uridine-5'-triphosphate. Formulation: Liquid. Solution in water. Purity: ≥93% (HPLC). Appearance: Yellow-green liquid and orange frozen solution. Use/Stability: Stable for at least one year after receipt when stored as recommended. Shipping: Shipped on Dry Ice. Long Term Storage: -20°C Excitation maximum: 496 nm Emission maximum: 520 nm. Handling: Protect from light. Avoid freeze/thaw cycles.
Enzo Life Sciences GW501516 (1mg). CAS: 317318-70-0
GW501516 is a specific agonist for PPARδ (EC50=1.1nM) with a 1'000-fold selectivity over the other human subtypes. Purity: ≥98% (HPLC). Appearance: White to off-white solid. Solubility: Soluble in DMSO (50mg/ml), dimethyl formamide (25mg/ml), 100% ethanol (12mg/ml) or methanol (50mg/ml); slightly soluble in water. Long Term Storage: +4°C.
Enzo Life Sciences BIO-PROBE Adenovirus probe (2µg)
The Adenovirus BIO-PROBE labeled probe is prepared by nick translation of cloned fragments of the adenovirus 5 genome. The fragments constitute a total of 20 kb of DNA, approximately 56% of the genome. The probe is specific for adenovirus and, in addition to hybridization to adenovirus 5 sequences, the probe will cross hybridize with adenovirus types 4, 7, 11, 20, 40 and 41 due to their considerable sequence homology. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis). Concentration: 20µg/ml. Formulation: Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA. Long Term Storage: -20°C.
Enzo Life Sciences BIO-PROBE 3'-Oligonucleotide labeling system reagent pack (25Reactions)
The procedure for labeling of DNA probes with a polynucleotide "tail" containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides. An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction. The BIO-PROBE 3'-Oligonucleotide Labeling System provides maximum flexibility for labeling oligonucleotides approximately 15 to 100 nucleotides in length. A standard reaction will label up to 100 picomoles. Applications: Southern Blot, Northern Blot, ISH (in situ hybridization), Dot Blot.
Enzo Life Sciences GW501516 (5mg). CAS: 317318-70-0
GW501516 is a specific agonist for PPARδ (EC50=1.1nM) with a 1'000-fold selectivity over the other human subtypes. Purity: ≥98% (HPLC). Appearance: White to off-white solid. Solubility: Soluble in DMSO (50mg/ml), dimethyl formamide (25mg/ml), 100% ethanol (12mg/ml) or methanol (50mg/ml); slightly soluble in water. Long Term Storage: +4°C.