Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit

Obtain 10–50 times yield of conventional in vitro transcription reactions

Supplier:  Invitrogen™ AM1348

Catalog No. AM1348

Description

Ambion mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15 - 35μg of 7-methyl guanosine capped RNA from 1μg of template DNA. Each kit includes sufficient reagents for 25 reactions.

• High yields of capped RNA—up to 39μg of RNA per reaction
• Extra convenience—cap analog-nucleotide mix provided
• Simplified reaction format
• Synthesized transcripts ideal for microinjection, in vitro translation, or transfection in a 20μL reaction during a 2 hour incubation, mMESSAGE mMACHINE High Yield Capped RNA Transcription will yield up to 35μg of capped RNA
• ™
• 10–50 times yield obtained with conventional in vitro transcription reactions
• Ratio of cap analog to GTP has been optimized to allow compromise between yield (15 - 35μg) and proportion of transcripts that are capped (80%)
• Contain a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
• Optimized for use with polymerases included in kit
• Contains 57U of cap analog

Specifications

• Content And Storage: T3 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Format: Kit
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• Product Line: Ambion™, mMESSAGE mMACHINE™
• Quantity: 25 Reactions
• Shipping Condition: Dry Ice
• Starting Material: PCR Products, Linearized DNA plasmid

• Final Product Type: Synthetic mRNA, mRNA
• Sample Type: mRNA, Synthetic mRNA
• No. of Reactions: 25 Reactions
• Product Type: T3 In Vitro Transcription kit
• Promoter: T3
• Yield: 20-30 μg

For Research Use Only. Not for use in diagnostic procedures.