Z-Competent E.coli Transformation KitThe Z-Competent E. coli Transformation Kit is a simple, highly efficient method for preparing cells for transformation. No heat shock or any other manipulation is required. Simply grow cells in SOB medium, wash and resuspend the cells in the Competent Buffer, then the cells are ready to receive DNA. Routinely produces 2 x 108 to 1 x 109 transformants per 1µg supercoiled pUC19 DNA. Competent cells can be used immediately or stored for later use. 300 transformation preparations