PCR Equipment and Supplies
PCR Equipment and Supplies
Enzo Life Sciences BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System (10Reactions)
The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis. This kit has been improved to yield sufficient aRNA quantity for most microarray platforms (10 µg minimum) with total RNA input of as low as 20 ng and up to 500 ng. Entire amplification reaction can be performed in a single tube and the biotin-labeled aRNA can be purified using either magnetic beads or purification columns. The BIOARRAY™ Low Input aRNA Amplification and Biotin Labeling System has been optimized for superior performance with reduced variability and improved reproducibility and data quality. The complete system is composed of reagents for cDNA synthesis and in vitro transcript labeling.
Enzo Life Sciences Fluorescein-12 ddUTP (25nmol)
Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies. Alternative name: Fluorescein-12-2',3'-dideoxyuridine-5'-triphosphate. Concentration: 1mM. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water. Long Term Storage: -20°C. Excitation maximum: 496 nm. Emission maximum: 520 nm.
Enzo Life Sciences 20X SSC (250ml)
Saline-Sodium Citrate (SSC) Concentrate can be used in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blottings. SSC provides high ionic strength to optimize the rate of probe annealing to the target nucleic acid. SSC is used in hybridization cocktails containing DNA and RNA probes for the analysis of unknown sequences and to locate desired genes and gene fragments. SSC is also used to control stringency for washing steps during in situ hybridization. Packet of salts yields 250mL of 20X SSC buffer. Alternative name: Saline-Sodium Citrate. Formulation: Lyophilized. Long Term Storage: Ambient. Applications: IF, Fluorescence microscopy FISH.
Enzo Life Sciences AMPIGENE 1-Step RT-PCR Kit (1000 Reactions)
AMPIGENE 1-Step RT-PCR Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. Applications: PCR.
Enzo Life Sciences AMPIGENE Taq Mix (200Reactions)
AMPIGENE Taq Mix uses the latest developments in polymerase technology and buffer chemistry to optimize PCR. The AMPIGENE Taq Mix is a robust mix for routine PCR applications including genotyping, screening, and library construction. An optimized buffer system allows efficient amplification under fast and standard cycling conditions. Applications: PCR.
Enzo Life Sciences ODN 1668 (TLRGRADE™) (synthetic) (BULK) (1mg)
CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9). Formulation: Lyophilized. Sterile. Long Term Storage: +4°C.
Enzo Life Sciences AMPIGENE 1-Step RT-PCR Kit (200 Reactions)
AMPIGENE 1-Step RT-PCR Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. Applications: PCR.
Enzo Life Sciences AMPIGENE Taq Mix (1000Reactions)
AMPIGENE Taq Mix uses the latest developments in polymerase technology and buffer chemistry to optimize PCR. The AMPIGENE Taq Mix is a robust mix for routine PCR applications including genotyping, screening, and library construction. An optimized buffer system allows efficient amplification under fast and standard cycling conditions. Applications: PCR.
Enzo Life Sciences AMPIGENE HS Taq Mix (40Reactions)
AMPIGENE HS Taq Mix uses the latest developments in polymerase technology and buffer chemistry to optimize and increase PCR sensitivity. The AMPIGENE HS Taq Mix is a robust mix using hot-start technology to increase sensitivity for PCR applications including genotyping, multiplex PCR, screening, library construction, colony PCR and PCR direct from blood and urine. An optimized buffer system allows efficient amplification under fast and standard cycling conditions. The AMPIGENE HS Taq Mix is resistant to PCR inhibitors and suitable for direct PCR from unprocessed samples: bacterial culture, bacterial colonies, blood, and urine. Applications: PCR.
Enzo Life Sciences AMPIGENE dNTP Mix (500µl)
AMPIGENE dNTP Mix is an ultrapure mix of deoxyribonucleotide triphosphates to use as building blocks for a wide variety of PCR applications. It can withstand multiple freeze-thaw cycles and shown to retain 95% dNTPs in triphosphate form after 5 weeks at room temperature. AMPIGENE dNTP Mix has been tested on many molecular biology applications including standard PCR, fast PCR, 1-step PCR, high-fidelity PCR, real-time PCR, lon-range PCR, MDA, RDA, LAMP, and DNA sequencing. Long Term Storage: -20°C. Applications: PCR.
Enzo Life Sciences AMPIGENE 1-Step RT-PCR Kit (50 Reactions)
AMPIGENE 1-Step RT-PCR Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. Applications: PCR.
Enzo Life Sciences AMPIGENE HS Taq Mix (1000Reactions)
AMPIGENE HS Taq Mix uses the latest developments in polymerase technology and buffer chemistry to optimize and increase PCR sensitivity. The AMPIGENE HS Taq Mix is a robust mix using hot-start technology to increase sensitivity for PCR applications including genotyping, multiplex PCR, screening, library construction, colony PCR and PCR direct from blood and urine. An optimized buffer system allows efficient amplification under fast and standard cycling conditions. The AMPIGENE HS Taq Mix is resistant to PCR inhibitors and suitable for direct PCR from unprocessed samples: bacterial culture, bacterial colonies, blood, and urine. Applications: PCR.
Enzo Life Sciences AMPIGENE qPCR 1-Step Probe Kit Hi-ROX (200Reactions)
AMPIGENE qPCR 1-Step Probe Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Probe Kits are designed for use on a variety of probes including: Taqman, Molecular Beacons, and Scorpion probes. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Probe Kits come with either Lo-ROX, Hi-ROX, or No-ROX concentration. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.) Applications: PCR.
Enzo Life Sciences AMPIGENE qPCR 1-Step Probe Kit No-ROX (200Reactions)
AMPIGENE qPCR 1-Step Probe Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Probe Kits are designed for use on a variety of probes including: Taqma , Molecular Beacons, and Scorpion probes. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Probe Kits come with either Lo-ROX, Hi-ROX, or No-ROX concentration. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.) Applications: PCR.
Enzo Life Sciences EPIXTRACT DNA Isolation Kit for Plasma/Serum (100Reactions)
EPIXTRACT DNA Isolation Kit for Plasma/Serum provides a simple method to isolate DNA from plasma, serum, and body fluids. Assay Time: <15 min.