Nucleic Acid Molecular Weight Markers
Nucleic Acid Molecular Weight Markers
New England Biolabs, Inc. Quick-Load Purple 1 kb DNA Ladder – 1.25 ml
Quick-Load Purple 1 kb DNA Ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing one tracking dye. Tracking dye does not leave a shadow under UV light. Quick-Load Purple 1 kb DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 10 kb. The 3 kb fragment has increased intensity to serve as reference band.
New England Biolabs, Inc. MidRange PFG Marker – 0.05 ml
MidRange PFG Marker consists of concatemers of DNA isolated from the bacteriophage (cI857 ind1 Sam7) mixed with XhoI digested DNA embedded in 1% LMP agarose and supplied in a GelSyringe dispenser. XhoI produces fragments of 15.0 and 33.5 kb. These fragments anneal to and form concatemers with intact DNA. It is designed for use as a size marker for pulsed field gel electrophoresis (PFG). Size range: 15-291 kb.
New England Biolabs, Inc. Quick-Load Purple 50 bp DNA Ladder – 1.25 ml
Quick-load Purple 50 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light. The Quick-Load Purple 50 bp DNA Ladder consists of a proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 50 to 1,350 base pairs. The 200 and 500 bp bands have increased intensity to serve as reference points.
New England Biolabs, Inc. Quick-Load Purple 100 bp DNA Ladder – 3.75 ml
Quick-Load Purple 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing one tracking dye. Tracking dye does not leave a shadow under UV light. Quick-Load Purple 100 bp DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 1,517 bp. The 500 and 1,000 bp bands have increased intensity to serve as reference bands.
New England Biolabs, Inc. TriDye™ Ultra Low Range DNA Ladder – 1.25 ml
TriDye Ultra Low Range DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing three tracking dyes which serve as visual aids to monitor the progress of migration during native agarose and polyacrylamide gel electrophoresis. The DNA ladder contains 13 bands ranging from 10 bp to 700 bp, most of which are generated from the digestion of a proprietary plasmid with restriction enzymes. The 50 bp and 300 bp bands have increased intensity to serve as reference bands. The ladder is suitable for use with for use with both native polyacrylamide and agarose gel electrophoresis.
New England Biolabs, Inc. Quick-Load 1 kb Plus DNA Ladder – 1.25 ml
Quick-Load 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands. NEB also offers a Quick-Load version of this ladder with purple dye.
New England Biolabs, Inc. Low Range ssRNA Ladder – 100 gel lanes
The Low Range ssRNA Ladder is a set of 6 RNA molecules produced by in vitro transcription of a mixture of 6 linear DNA templates. The ladder sizes are: 1000, 500, 300, 150, 80 and 50 bases. The 300 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing polyacrylamide-urea gels, and on denaturing or native agarose gels.
New England Biolabs, Inc. 1 kb Plus DNA Ladder – 0.1 ml
A single convenient ladder for 100 bp up to 10 kb (100 bp and 1 kb ladder in one!). A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference points. The approximate mass of DNA in each of the bands is provided (assuming a 1.0 ug load) for approximating the mass of DNA in comparably intense samples of similar size. Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS. NEB also offers a Quick-Load version of this ladder with purple dye.
New England Biolabs, Inc. PCR Marker – 0.1 ml
The PCR Marker consists of a proprietary plasmid that is digested to completion with appropriate restriction enzymes to yield 5 double-stranded DNA bands suitable for use as molecular weight standards for agarose and acrylamide gel electrophoresis. The digested DNA includes fragments ranging from 50-766 base pairs. Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
New England Biolabs, Inc. Rapid™ PNGase F (non-reducing format) – 50 reactions
Developed for proteomic applications, Rapid PNGase F (non-reducing format) enables complete and rapid deglycosylation, while preserving disulfide bonds. This facilitates high throughput proteomics applications and methods for antibody characterization by mass spectrometry, such as intact mass analysis. Rapid PNGase F (non-reducing format) combines the advantages of Rapid PNGase F (fast processing time), with non-reducing conditions, preserving quaternary structure.
New England Biolabs, Inc. Quick-Load Purple 1 kb DNA Ladder – 3.75 ml
Quick-Load Purple 1 kb DNA Ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing one tracking dye. Tracking dye does not leave a shadow under UV light. Quick-Load Purple 1 kb DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 10 kb. The 3 kb fragment has increased intensity to serve as reference band.
New England Biolabs, Inc. ssRNA Ladder – 25 gel lanes
The ssRNA Ladder is a set of 7 RNA molecules produced by in vitro transcription of a mixture of 7 linear DNA templates. The ladder sizes are: 9000, 7000, 5000, 3000, 2000, 1000 and 500 bases. The 3000 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing or native agarose gels.
New England Biolabs, Inc. Lambda DNA-Hind III Digest – 1.5 ml
The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis. The approximate mass of DNA in each of the bands is provided (assuming a 1.0 ug load) for approximating the mass of DNA in comparably intense samples of similar size. Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
New England Biolabs, Inc. PhiX174 DNA-Hae III Digest – 0.05 ml
The HaeIII digest of X174 yields 11 fragments suitable for use as molecular weight standards for agarose gel electrophoresis. Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.
New England Biolabs, Inc. TriDye™ 1 kb Plus DNA Ladder – 1.25 ml
TriDye 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference points.