Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
Cytiva Whatman™ Gel Blotting Paper Grade GB003
A thick paper ideal for lysis/denaturation of colony or plaque lifts and Western blots
Invitrogen™ eBioscience™ IHC /ICC Blocking Buffer - Low Protein
Designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. IHC /ICC Blocking Buffer, Low Protein is recommended for tissues and antibodies with average levels of background staining.
Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Cytiva Amersham™ Hyperfilm™ ECL
Use with all blue and green light chemiluminescent systems. Cytiva Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.
Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls
Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
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MilliporeSigma™ Immobilon™-FL PVDF Membranes
Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45 μm pores and have lower background fluorescence than other membranes. Available in 10 and 20 cm squares and a 3.75 m roll.
Invitrogen™ Nitrocellulose/Filter Paper Sandwich, 0.2 μm, 8.3 x 7.3 cm
Ideal for blotting proteins and nucleic acids
Invitrogen™ Nitrocellulose/Filter Paper Sandwich, 0.45 μm, 8.3 x 7.3 cm
Ideal for blotting proteins and nucleic acids
Invitrogen™ XCell II™ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Thermo Scientific™ HisProbe™-HRP Conjugate
Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.
Thermo Scientific™ Pierce™ Recombinant Protein A/G
Detect antibodies, especially IgG isotypes, from many different species hosts with this purified, alkaline phosphatase- or peroxidase-conjugated recombinant Protein A/G.