Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Catalog Number	Mfr. No.	No. of Reactions	Gel Compatibility
A44717	Invitrogen™ A44717	A standard kit will label 10 mini gels or 10 mini gel-sized membranes	Mini Precast Protein Gels, Midi Precast Protein Gels
A44449	Invitrogen™ A44449	A standard kit will label 40 mini gels or 40 mini gel-sized membranes	Any Gel Type and Gel Chemistry

Description

• Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
• Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
• Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
• Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
• Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
• Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.

Specifications

• Description: The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
• Product Line: Invitrogen™
• Run Time: 10 minutes
• Product Type: Protein Labeling Reagent

• Membrane Compatibility: PVDF, Nitrocellulose
• For Use With (Equipment): Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
• Shipping Condition: Dry Ice
• For Use With (Application): Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection

Safety and Handling

ShelfLife : At least 2 years from receipt

For Research Use Only. Not for use in diagnostic procedures.