Staining Reagents
Staining Reagents
STEMCELL Technologies LysoBrite™ Lysosome Dye, Red DND-99, Size: 500 Tests
LysoBrite™ dyes are fluorescent dyes that selectively stain lysosomes and other acidic organelles. Due to their hydrophobic nature, LysoBrite™ dyes permeate live cells and selectively accumulate in the lysosomes via the lysosome’s pH gradient. The fluorescence property of these dyes significantly increases within the lysosome’s acidic environment; this fluorescence can be measured using flow cytometry and fluorescence imaging. Due to their selective fluorescence inside lysosomes, LysoBrite™ dyes provide low background and high contrast during imaging. They can be used with proliferating and non-proliferating cells, and are suitable for suspension and adherent cells.
STEMCELL Technologies Propidium Iodide, Size: 10 mg
Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases. It is widely used as a counterstain to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using blue (488 nm), green (532 nm), or yellow-green (561 nm) laser lines, with detection in the FL2 or FL3 channels. PI is used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome analysis. It is also commonly used in quantitative DNA assays.
STEMCELL Technologies Annexin V Binding Buffer, Size: 50 mL
Annexin V Binding Buffer is an isotonic buffer used in conjunction with Annexin V dyes (e.g. Catalog 100-0329) and either 7-AAD (Catalog 75001) or Propidium Iodide (Catalog 75002). This buffer contains an optimal concentration of calcium that facilitates the binding of Annexin V to phosphatidylserine on the cell surface which is essential for the detection of early-stage cell apoptosis by flow cytometry.
STEMCELL Technologies Carmine Alum, Size: 100 mL
Carmine Alum is used for the whole mount staining of mouse mammary gland. This product contains carmine and aluminum potassium sulfate in water.