Recombinant Proteins
Recombinant Proteins
STEMCELL Technologies Human Recombinant IL-1 alpha, Size: 10 µg
Interleukin 1 alpha (IL-1α) is a member of the IL-1 family and a dual-function cytokine. Both the unprocessed precursor and a processed IL-1α protein signal through IL-1 receptor type 1 (IL-1R1). Various cells, including keratinocytes, thymic epithelium, hepatocytes, endothelial cells, fibroblasts, and the epithelial cells of mucous membranes, have high levels of intracellular IL-1α precursor. The precursor is also expressed on the surface of monocytes and B lymphocytes (Netea et al.). IL-1α recruits infiltrating cells to a site of injury during necrosis and plays an important role during processes of sterile inflammation (Cohen et al.; Rider et al.). During hypoxia, IL-1α contributes to angiogenesis (Carmi et al.). Studies in mice show that IL-1α is produced by microglia-like cells after ischemic brain injury, which contributes to the inflammation (Luheshi et al.).
STEMCELL Technologies Human Recombinant GM-CSF (E. coli-expressed), Size: 500 µg
Use granulocyte-macrophage colony-stimulating factor (GM-CSF) to induce the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages (Barreda et al. Dev Comp Immunol, 2004). Recombinant human GM-CSF also promotes the production of myeloid cells of the granulocytic and monocytic lineages in vivo. It has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. In combination with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, GM-CSF also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). Human recombinant GM-CSF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 200 pg/mL; endotoxin levels verified ≤ 1.0 EU/μg protein. Alternative names: Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoitin-alpha. Visit STEMCELL.com for more information.
STEMCELL Technologies Human Recombinant GM-CSF (CHO-expressed), Size: 10 µg
Use granulocyte-macrophage colony-stimulating factor (GM-CSF) to induce the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages (Barreda et al. Dev Comp Immunol, 2004). Recombinant human GM-CSF also promotes the production of myeloid cells of the granulocytic and monocytic lineages in vivo. GM-CSF has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. In combination with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). Human recombinant GM-CSF (CHO-expressed) from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 0.2 ng/mL; endotoxin levels verified ≤ 0.2 EU/μg protein. Alternative names: Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoitin-alpha. Visit STEMCELL.com for more information.
STEMCELL Technologies Mouse Recombinant IL-3, Size: 10 µg
Use interleukin 3 (IL-3) to promote the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages (Broughton et al. Immunol Rev, 2012). Produced by activated T cells, IL-3 is a species-specific pleiotropic cytokine that has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators. When IL-3 binds to heterodimeric receptors containing the alpha subunit and one of either beta subunits, it activates JAK/STAT, MAPK, and PI3K signaling pathways (Scott & Begley. Int J Biochem Cell Biol, 1999). Mouse recombinant IL-3 from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 0.25 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: FGFA, Fibroblast growth factor-10, Keratinocyte growth factor-2. Visit STEMCELL.com for more information.
STEMCELL Technologies Human Recombinant GM-CSF (E. coli-expressed), Size: 100 µg
Use granulocyte-macrophage colony-stimulating factor (GM-CSF) to induce the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages (Barreda et al. Dev Comp Immunol, 2004). Recombinant human GM-CSF also promotes the production of myeloid cells of the granulocytic and monocytic lineages in vivo. It has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. In combination with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, GM-CSF also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). Human recombinant GM-CSF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 200 pg/mL; endotoxin levels verified ≤ 1.0 EU/μg protein. Alternative names: Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoitin-alpha. Visit STEMCELL.com for more information.
STEMCELL Technologies Human Recombinant GM-CSF, ACF, Size: 5 µg
Use granulocyte-macrophage colony-stimulating factor (GM-CSF) to induce the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages (Barreda et al. Dev Comp Immunol, 2004). Recombinant human GM-CSF also promotes the production of myeloid cells of the granulocytic and monocytic lineages in vivo. GM-CSF has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. In combination with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). Human recombinant GM-CSF from STEMCELL is animal component-free and comes lyophilized with ≥ 95% purity; EC50 ≤ 200 pg/mL; endotoxin levels verified ≤ 1.0 EU/μg protein. Alternative names: Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoitin-alpha. Visit STEMCELL.com for more information.
STEMCELL Technologies Mouse Recombinant LIF, Size: 50 µg
Use leukemia inhibitory factor (LIF) to regulate a variety of developmental functions. LIF is an interleukin 6 class cytokine that regulates proliferation, invasion, and differentiation of trophoblasts following blastocyst attachment (Suman et al. JAK-STAT, 2014). Upon binding of LIF to LIF receptor (LIFR), LIFR associates with gp130 and activates JAK/STAT and MAPK signaling (Suman et al.). LIFR activation of STAT3 is essential for maintaining the mouse embryonic stem cell phenotype (Niwa et al. Genes Dev, 1998). LIF also supports hematopoiesis and immune modulation (Nasef et al. Cell Immunol, 2008) Mouse recombinant LIF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 0.01 ng/mL; endotoxin levels verified ≤ 0.2 EU/μg protein. Alternative names: Cholinergic differentiation factor, D Factor, Differentiation-inducing factor, Differentiation inhibitory activity, Emfilermin, Hepatocyte-stimulating factor III, Leukemia inhibitory factor, MLPLI.
STEMCELL Technologies Human Recombinant G-CSF, Size: 100 µg
Use granulocyte colony-stimulating factor (G-CSF) to induce the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola. J Cell Physiol, 1983). This cytokine is involved in the mobilization of hematopoietic stem cells from the bone marrow into the periphery through a variety of mechanisms. Examples include transiently reducing SDF-1 expression (Petit et al. Nat Immunol, 2002), activating metalloproteases that cleave VCAM-1 (Levesque et al. Blood, 2001), and releasing norepinephrine from the sympathetic nervous system (Katayama et al. Cell, 2006). Binding of G-CSF to its receptor also leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways. Human recombinant G-CSF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 0.05 ng/mL; endotoxin levels verified ≤ 1.0 EU/μg protein. Alternative names: Colony-stimulating factor 3, CSF-3, MGI-1G, Pluripoietin.
STEMCELL Technologies Mouse Recombinant G-CSF, Size: 10 µg
Use granulocyte colony-stimulating factor (G-CSF) to induce the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola. J Cell Physiol, 1983). This cytokine is involved in the mobilization of hematopoietic stem cells from the bone marrow into the periphery through a variety of mechanisms. Examples include transiently reducing SDF-1 expression (Petit et al. Nat Immunol, 2002), activating metalloproteases that cleave VCAM-1 (Levesque et al. Blood, 2001), and releasing norepinephrine from the sympathetic nervous system (Katayama et al. Cell, 2006). Binding of G-CSF to its receptor also leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways. Mouse recombinant G-CSF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 0.05 ng/mL; endotoxin levels verified ≤ 1.0 EU/μg protein. Alternative names: Colony-stimulating factor 3, CSF-3, MGI-1G, Pluripoietin.
STEMCELL Technologies Mouse Recombinant SCF, Size: 1000 µg
Use stem cell factor (SCF) to promote cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. SCF is an early-acting cytokine that activates PI3K, JAK/STAT, and MAPK pathways upon binding to its receptor. Together with other cytokines like thrombopoietin and Flt3/Flk-2 Ligand, SCF is used to promote expansion of primitive hematopoietic stem cells and multipotent progenitor cells in culture (Martin et al. Cell, 1990; Kent et al. Clin Cancer Res, 2008.). In combination with various growth factors and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy VC. Blood, 1997). Mouse recombinant SCF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 20 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: Kit ligand, Mast cell growth factor, Steel factor, Stem cell factor.
STEMCELL Technologies Human Recombinant SCF, ACF, Size: 1000 µg
Use stem cell factor (SCF) to promote cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. SCF is an early-acting cytokine that activates PI3K, JAK/STAT, and MAPK pathways upon binding to its receptor. Together with thrombopoietin and Flt3/Flk-2 Ligand, SCF is used to promote expansion of primitive hematopoietic stem cells and multipotent progenitor cells in culture (Martin et al. Cell, 1990; Kent et al. Clin Cancer Res, 2008.). In combination with various growth factors and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy VC. Blood, 1997). Human recombinant SCF from STEMCELL is animal component-free and comes lyophilized with ≥ 95% purity; EC50 ≤ 15 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: Kit ligand, Mast cell growth factor, Steel factor, Stem cell factor.
STEMCELL Technologies Rat Recombinant TNF-alpha, Size: 50 µg
Use tumor necrosis factor-alpha (TNF-α) to activate the NF-κB, MAPK, and PI3K/AKT pathways. TNF-α is a pro-inflammatory cytokine that stimulates the expression of E- and P-selectins, facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová & Hošek. Inflamm Res, 2013). It also stimulates anti-tumor immunosuppressive responses, vasodilation, and edema formation. TNF-α is primarily produced by activated T cells and macrophages in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, such as B cells, NK cells, mast cells, neutrophils, dendritic cells, endothelial cells, cardiomyocytes, and fibroblasts. Rat recombinant TNF-α from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 0.05 ng/mL; endotoxin levels verified ≤ 0.2 EU/μg protein. Alternative names: Cachectin, Cytotoxin, Differentiation-inducing factor, DIF, Necrosin, TNFA, TNFSF2, Tumor necrosis factor.
STEMCELL Technologies Human Recombinant SCF, ACF, Size: 100 µg
Use stem cell factor (SCF) to promote cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. SCF is an early-acting cytokine that activates PI3K, JAK/STAT, and MAPK pathways upon binding to its receptor. Together with thrombopoietin and Flt3/Flk-2 Ligand, SCF is used to promote expansion of primitive hematopoietic stem cells and multipotent progenitor cells in culture (Martin et al. Cell, 1990; Kent et al. Clin Cancer Res, 2008.). In combination with various growth factors and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy VC. Blood, 1997). Human recombinant SCF from STEMCELL is animal component-free and comes lyophilized with ≥ 95% purity; EC50 ≤ 15 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: Kit ligand, Mast cell growth factor, Steel factor, Stem cell factor.
STEMCELL Technologies Human Recombinant DKK-1, Size: 1000 ug
Use dickkopf-related protein 1 (DKK-1) to drive cardiac and retinal differentiation from induced pluripotent stem (iPS) cells (Lian et al. Proc Natl Acad Sci USA 2012). DKK-1 is a secreted protein that inhibits the canonical WNT pathway by competitive binding to low-density lipoprotein receptors (LRP)-5 and 6 (Niehrs. Oncogene 2006). This protein regulates embryonic development (Lieven O et al. Dev Biol 2010) and has been shown to support the generation of myeloid-derived suppressor cells (MDSCs) (D’Amico et al. J Exp Med 2016). DKK-1 from thrombocytes also induces Th2 cell polarization and potentiates Th2 cell cytokine expression (Chae et al. Immunity 2016). Human recombinant DKK-1 from STEMCELL contains a His-residue tag at the carboxyl end of the polypeptide chain and comes lyophilized with ≥ 95% purity EC50 4 μg/mL endotoxin levels verified 0.2 EU/μg protein. Alternative names Dickkopf-1 Dickko
STEMCELL Technologies Human Recombinant Betacellulin, Size: 1000 µg
Use betacellulin to induce neural stem cell proliferation and prevent spontaneous differentiation in culture through ERK and AKT pathways. Betacellulin is a member of the epidermal growth factor (EGF) family, and signals through EGF receptor and ERBB4. It stimulates the expansion of neural stem cells, transit-amplifying cells, and neuroblasts derived from subventricular zone and dentate gyrus (Gómez-Gaviro et al. Proc Natl Acad Sci USA, 2012). Betacellulin also induces the proliferation of pancreatic cancer cell lines (Kawaguchi et al. Int J Oncol, 2000) and down-regulates E-cadherin expression in ovarian cancer cell lines via MEK/ERK1/2 and PI3K/AKT signaling pathways, thus increasing cell migration (Zhao et al. Oncotarget, 2016). Betacellulin is expressed in pancreatic α cells, β cells, and duct cells. Human recombinant betacellulin from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 0.01 ng/mL; endotoxin levels verified ≤ 0.2 EU/μg protein. Alternative names: BTC.