Western Blotting

Cytiva™ Amersham™ Western blotting products deliver reliable protein identification, meaningful data, and reproducible results time after time.

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ECL Detection Reagents

Cytiva™ Amersham™ ECL™ Start is a chemiluminescent kit designed for routine Western blotting applications. Combined with Cytiva Amersham ECL Select, the most sensitive formulation Cytiva offers for qualitative and quantitative analysis, you’ll have a solution optimized for your research needs.

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Amersham Hybond and Protran Membrane

Cytiva offers an extensive range of blotting membranes made of nitrocellulose, PVDF, or nylon with pore sizes from 0.1 to 0.45 µm to suit your applications. These are the Cytiva™ Hybond™, BA83, and BA85 membranes you’ve known for years, now available in longer roll lengths.

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Amersham Hyperfilm

Cytiva™ Amersham™ Hyperfilm™ ECL™ low-background films are suitable for use with all blue and green light chemiluminescent systems and validated for use with Cytiva Amersham ECL, Cytiva Amersham ECL Advance, and Cytiva™ CDP-Star™ reagents.

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Featured Products

Protein Purification

Choose from the Cytiva™ portfolio to support your protein purification needs. From Sephadex, the resin that revolutionized protein purification, to the newest magnetic bead-based technology, you'll find products to support a broad range of chromatography media and resin methods. This includes affinity purification, gel filtration, desalting, ion exchange, and hydrophobic interaction.

  • Loose media up to 500 g
  • Cytiva™ HiTrap™, SpinTrap™, GraviTrap™, and Tricorn™ chromatography columns
  • Pre-packed columns for convenience and efficiency

Featured Media/Resins

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HiTrap rProtein A FF Columns

Quick, convenient preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum, and ascites.

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HiTrap Desalting and IEX Columns

With an optimized design for desalting buffer exchange and IEX chromatography, Cytiva HiTrap columns provide targeted screenings and ready-to-use formats.

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HisTrap FF Crude Prepacked Columns

Achieve high-binding efficiency, speed, and convenience with columns optimized for purification of histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap FF crude prepacked columns include additional filtration for difficult samples.

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Filtration

Filtration is an indispensable step in sample preparation and many analytical methods. Cytiva™ Whatman™ products provide quality, consistency, reliability, and convenience to help your lab run smoothly. With a large variety of sizes, types, and specifications available, you can be sure to find a filter that fits your application, equipment, and workflow.

  • Cellulose, glass, and quartz fiber filters
  • Membrane filters for microbiological applications
  • Syringe filtration devices
  • Polycap capsule filters
  • Sterile and non-sterile options
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Filtration

The Cytiva portfolio includes a complete range of cellulose filter papers, glass, and quartz fiber filters. For the retention of very small particles or for microbiological purposes, Cytiva recommends its offering of membrane filters with pore sizes ranging from 0.015 µm to 12 µm.

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Capsulated Filters

Cytiva syringe filters are available in different diameters, pore sizes, and membrane types. Sterile or nonsterile versions come with or without prefilters and syringeless filters. Cytiva™ Whatman™ Polycap™ capsule filters are available in three sizes, either sterile or nonsterile, and with effective filtration surface areas ranging from 500 cm2 to 1,500 cm2.

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HyClone Cell Culture

Cytiva™ HyClone™ serum, media buffers, and process liquids have helped advance drug discovery and bioproduction processes since 1967 and continue to deliver quality and consistency.

Media

Select from a complete line of Cytiva HyClone classical, specialty, and transfection media for your cell culture applications. You can support the growth of CHO SF9, HEK293, hybridoma, myeloma, insect, NSO, and vial vaccine cell lines.