Cytiva HiTrap™ rProtein A FF Columns

Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.

Catalog Number	Mfr. No.	Quantity	Flow Rate
45-000-262	Cytiva 17507901	5 x 1 mL	<4 mL/min.
45-000-263	Cytiva 17507902	2 x 1 mL	<4 mL/min.
45-000-265	Cytiva 17508002	5 x 5 mL	<20 mL/min.
45-000-264	Cytiva 17508001	1 x 5 mL	<20 mL/min.

Description

• Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
• Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
• Average particle size: 90μm
• Designed for use with a syringe, a peristaltic pump, or a chromatography system
• Available as 1mL and 5mL columns

Media

• Ligand: Recombinant protein A (E.coli)
• Matrix: Highly cross-linked 4% agarose
• Binding capacity: ∽50mg human IgG/mL
• pH stability working range: 3 to 10

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Order Info

1mL and 5mL columns are packed with 1mL and 5mL of rProtein A Sepharose Fast Flow, respectively.

Specifications

• Binding Capacity: ≈50 mg Human IgG/mL
• For Use With (Application): Chromatography System, Manual
• For Use With (Equipment): For syringe, peristaltic pump or a chromatography system
• Content And Storage: 4°C to 30°C, 20% Ethanol