Applied Biosystems™ TaqMan™ Comprehensive Microbiota Control

The TaqMan Comprehensive Microbiota Control contains a linearized multi-target plasmid pool with microbial target sequences commonly used on Custom OpenArray panels. The control has targets relevant to many microbial profiling applications, including respiratory tract, urinary tract, vaginal microbiota, and antibiotic resistance research.

Supplier:  Applied Biosystems™ A50382

Catalog No. A50382

Description

The TaqMan Comprehensive Microbiota Control control can be included as a positive control for panel-specific amplification. It is available in two concentrations: 1 x 10⁵ and 5 x 10⁷ copies/μL. The higher concentration can be used to determine parameters necessary for analytical validation (e.g., limit of detection and Ct cut-off values).

The plasmid also contains target sequences for TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control, TaqMan Universal Extraction Control Organism (B. atrophaeus), and the human RNase P RPPH1 gene. During real-time PCR, the control can be used as a stand-alone sample to verify assay performance and to help with troubleshooting.

Note: This product will be updated on an annual basis to ensure coverage of the most popular microbial targets, and performance will be measured to ensure consistency between versions.

Version History:

Version 1 of this product was first available in April 2021 and contained 471 microbial assay targets.

Version 2 became available in October 2021 and contains an additional 83 microbial assay targets.

Specifications

• Content And Storage: 1000 μL at a concentration of 1 x 10⁵ copies/μL
• Format: Tube
• Shipping Condition: Dry ice
• Volume: 1000 μL

• Detection Method: Primer-probe
• Product Line: TrueMark
• Concentration: 1 x 10⁵ copies/μL

Safety and Handling

ShelfLife : 2 years