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Real Time PCR Products

Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Reagents and Kits (621)
Real Time PCR Master Mixes (519)
Real Time PCR Assays and Arrays (288)

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1 – 15 of 1,154 results
1

Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Content And Storage SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Product Type Master Mix
Form Frozen
Reaction Speed Fast or Standard
Label or Dye SYBR Green,FAM
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method qPCR,2-step RT-qPCR,RT-qPCR
Detection Method SYBR
2

Invitrogen™ ROX Reference Dye

ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.

EDGE ENCOMPASS_PREFERRED
Content And Storage Store in freezer (-5° to -30°C).
Label or Dye ROX
Detection Method Primer-probe
3

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type RNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Pathogen Detection,Virus Detection
PCR Method 1-step RT-qPCR
Product Line Platinum™, SuperScript™
Detection Method Primer-probe
4

Invitrogen™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
Product Type One-Step qRT-PCR Kit
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type RNA
Reaction Speed Standard
For Use With (Application) Real Time PCR (qPCR)
PCR Method 1-step RT-qPCR
Product Line Platinum™, SYBR™, SuperScript™
Detection Method SYBR
5

Invitrogen™ SYBR GreenER™ qPCR SuperMix Universal

Incorporate the latest high-performance technology to provide the most reliable gene expression data on a variety of platforms including ABI 7500, Corbett Rotor-Gene™ etc.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7500 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P, MJ Chromo4, MJ Opticon
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum™, SYBR™ GreenER™
Detection Method SYBR
6

Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Wet Ice
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase ArrayScript™ UP
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type RNA
Reaction Speed Standard
Label or Dye SYBR Green
Run Time Standard
Product Line RNA-to-CT™, SYBR™
7

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Sample Type RNA
Reaction Speed Standard
Product Line AgPath-ID™, Ambion™
8

Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, ROX

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

EDGE
GC-Rich PCR Performance High
Format Tube
Product Type 1-Step RT-qPCR Kit
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type RNA
For Use With (Application) Gene Expression,Pathogen Detection
PCR Method 1-step RT-qPCR
Product Line Verso
Detection Method SYBR
9

Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, low ROX

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

EDGE
GC-Rich PCR Performance High
Format Tube
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type RNA
PCR Method 1-step RT-qPCR
Product Line Verso
Detection Method SYBR
10

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility Block-Based qPCR Instruments
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute Blue
Detection Method SYBR
11

Thermo Scientific™ ROX Solution, 50 μM

ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX.

EDGE
Content And Storage Contains:
• 1 mL ROX Solution, 50 μM

Store at -20°C.
Label or Dye ROX
Detection Method Primer-probe
12

Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (VIC™/TAMRA™ probe, primer limited)

Human ACTB (Beta Actin) Endogenous Control (VIC™/TAMRA™ probe, primer limited)

EDGE ENCOMPASS_PREFERRED
13

Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)

Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)

EDGE ENCOMPASS_PREFERRED
14

Applied Biosystems™ Human HPRT1 (HGPRT) Endogenous Control (VIC™/MGB probe, primer limited)

Human HPRT1 (HGPRT) Endogenous Control (VIC™/MGB probe, primer limited)

EDGE ENCOMPASS_PREFERRED
15

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

EDGE ENCOMPASS_PREFERRED
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan™
Detection Method Primer-probe