Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Supplier:  Invitrogen™ A44449

Catalog No. A44449

Description

• Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
• Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
• Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
• Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
• Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
• Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.

Specifications

• Detection Method: Chemiluminescence, Fluorescence
• Product Line: Invitrogen™
• Membrane Compatibility: PVDF, Nitrocellulose
• No. of Reactions: A standard kit will label 40 mini gels or 40 mini gel-sized membranes
• For Use With (Equipment): Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
• Run Time: 10 minutes

• Content And Storage: • No-Stain activator, 800 μL, store at -20°C
  • No-Stain derivatizer, 800 μL, store at -20°C
  • No-Stain labeling buffer (20X), 2 x 20 mL, store at 15°C to 30°C
• Gel Compatibility: Any Gel Type and Gel Chemistry
• Description: The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
• Shipping Condition: Dry Ice
• Product Type: Protein Labeling Reagent
• For Use With (Application): Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection

Safety and Handling

ShelfLife : At least 2 years from receipt

For Research Use Only. Not for use in diagnostic procedures.