Invitrogen™ VCAM-1 (Soluble) Human ELISA Kit

For the quantitative detection of human sVCAM-1.

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sVCAM-1
Conjugate Mixture containing Biotin-Conjugate anti-human sVCAM-1 monoclonal antibody mixed with Streptavidin-HRP
Human sVCAM-1 Standard lyophilized, 200ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Catalog Number	Mfr. No.	Quantity
50-181-05	Invitrogen™ BMS232	96 Tests
50-181-07	Invitrogen™ BMS232TEN	10 x 96 Tests

Description

The Human Vascular Cell Adhesion Molecula-1 (Hu VCAM-1) ELISA quantitates Hu VCAM-1 in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VcAM-1. Principle of the method The Human VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

Specifications

• Accession Number: P19320
• Assay Sensitivity: 0.6 ng/mL
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD106,INCAM-100
• Intraassay CV: 3.1%
• Label or Dye: HRP
• Sample Volume: Amniotic Fluid, 100 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 100 μL
• Synonym: Vascular cell adhesion protein 1, CD106 antigen
• Test Time: 1 hr. 20 min.

• Assay Range: 3.15-100 ng/mL
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 7412
• Interassay CV: 5.2%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 2 hr. 10 min.

For Research Use Only