Invitrogen™ VCAM-1 (Soluble) Human ELISA Kit

For the quantitative detection of human sVCAM-1.

Supplier:  Invitrogen™ BMS232TEN

Catalog No. 50-181-07

Includes:

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sVCAM-1
• Conjugate Mixture containing Biotin-Conjugate anti-human sVCAM-1 monoclonal antibody mixed with Streptavidin-HRP
• Human sVCAM-1 Standard lyophilized, 200ng/mL upon reconstitution
• Control high, lyophilized
• Control low, lyophilized
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Blue-Dye
• Green-Dye
• Adhesive Films

Description

The Human Vascular Cell Adhesion Molecula-1 (Hu VCAM-1) ELISA quantitates Hu VCAM-1 in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VcAM-1. Principle of the method The Human VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

VCAM-1 (vascular cell adhesion protein 1, CD106, INCAM-110) is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases. VCAM-1 is distributed across non-leukocyte and leukocyte cells. VCAM-1 expression is reportedly correlated with adhesion of melanoma, Burkitt lymphoma, osteosarcoma, and kidney carcinoma but not colon carcinoma cells to endothelial cells which may determine the site of tumor metastasis. VCAM-1 is a member of the Ig superfamily of adhesion molecules, is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on unstimulated endothelial cells. VCAM-1 is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. VCAM-1 serves as a ligand for leukocyte integrin alpha-beta (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. VCAM-1 plays a role in tumor metastasis, and may also be involved in the development of atherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms of VCAM-1 have been described.

Specifications

• Accession Number: P19320
• Assay Sensitivity: 0.6 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD106,INCAM-100
• Intraassay CV: 3.1%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 2 hr. 10 min.

• Assay Range: 3.15-100 ng/mL
• Conjugate: Biotin
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 7412
• Interassay CV: 5.2%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Amniotic Fluid, 100 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 100 μL
• Synonym: Vascular cell adhesion protein 1, CD106 antigen
• Test Time: 1 hr. 20 min.

For Research Use Only