Applied Biosystems SeqStudio Genetic Analyzer

Designed for individual labs and core facilities, the Applied Biosystems™ SeqStudio™ Genetic Analyzer is an easy-to-use, compact benchtop system that streamlines and simplifies a diverse array of applications.

• Easy-to-use: Universal, all-in-one reusable cartridge, extends reagent use and replaces several hands-on steps; simply load your samples, click in your cartridge, and go
• Flexible: Bypass time-consuming batched sample setup and perform both Sanger sequencing and fragment analysis applications on the same plate during the same run
• Fast results: 30-minute run time
• Ideal for multi-user labs: One-of-a-kind cartridge-based system enables multiple users to run different experiments on the same platform

SeqStudio Genetic Analyzer Applications

Plasmid Sequencing

Accurately analyze subcloned inserts with read lengths ranging from <300 bp to >600 bp.

Species Identification

Identify bacterial 16s rRNA with access to the groundbreaking phylogenetic research work of Carl Woese et al.

CRISPR-Cas9 Gene Editing

Evaluate the efficiency of CRISPR events, quantify gene editing activity (target region analyzed using TIDE software), characterize the gene target in cell lines, assess gene copy number, and more.

Cell Line Authentication (CLA)

Confidently detect contaminating cell lines with sensitivity and accuracy.

SNP Genotyping

The Applied Biosystems™ SNaPshot™ Multiplex System saves time with built-in reporting of fragment analysis and by combining sequencing and fragment analysis on the same plate during the same run.

MLPA

Analyze human copy number variation (CNV) with Multiplex Ligation-Dependent Probe Amplification (MLPA™).

Oncology Research

Confirm next-generation sequencing.

Figure 8. Analysis of cell line contamination on the SeqStudio instrument.
HeLa cells and M4A4GFP cell suspensions were diluted to 5 x 105 cells/mL, mixed in the indicated proportions, and spotted onto NUCLEIC-CARD™ Sample Collection Device. Contaminating HeLa cells can be detected with high confidence on the SeqStudio instrument if they make up approximately 20% of a population; however, some alleles unique to HeLa can be detected if they make up as little as 10% of a population.

"The SeqStudio instrument was very easy to set up in the lab; it did not require us to have a lot of initial expertise because the instrument is extremely easy to handle. By using the RAS panel, we found very consistent results across the different platforms that we used, whether it was the Sanger platform or the NGS platform."

Dr. Luca Quagliata, Senior Director of the Contract R&D Unit at the Institute of Medical Genetics and Pathology, University Hospital of Basel, Switzerland