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Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells
Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Approved for shipment at Room Temperature or on Wet or Dry Ice |
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Promoter | T3, T7 |
Content And Storage | Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes Box 3: • One Shot OmniMAX 2 T1R chemically competent E. coli • S.O.C. Medium • pUC 19 Control DNA Store competent cells and pUC19 at -65 to -85°C. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | Zero Blunt TOPO |
Bacterial or Yeast Strain | OmniMAX2 |
For Use With (Application) | PCR Cloning |
Vector | pCR-XL-2-TOPO |
Product Line | TOPO™ |
Cell Type | Chemically Competent E. coli |
Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Blue/White Screening | Yes (lacZΔM15) |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | High Copy Plasmid |
Reduces Recombination | Yes (recA1) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Streptomycin) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | No |
Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli
The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Packaging)
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Only)
Designed for cloning PCR products directly from PCR reaction in 5 minutes
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MC1061/P3 Chemically Competent E. coli
MC1061/P3 Chemically Competent E. coli are used for highly efficient transformations that require the P3 episome for selection and maintenance of vectors encoding the tyrosine tRNA suppressor (synthetic supF gene, such as pCDM8, pcDNA1.1, or any other supF-containing vector).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli
MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Blue/White Screening | Yes (lacZΔM15) |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | Medium Efficiency (1 x 108 to 1 x 109 cfu/μg) |
High-throughput Compatibility | High |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | High Copy Plasmid |
Reduces Recombination | Yes (recA1) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Streptomycin) |
Product Type | Chemically Competent Cells |
Product Line | MultiShot™ |
Contains F' Episome | No |
Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli
One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Blue/White Screening | Yes (lacZΔM15) |
Cloning Unstable DNA | Not suitable for cloning unstable DNA |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Promoter | Trc, Tac, Lac |
Preparing Unmethylated DNA | No |
Reduces Recombination | Yes (recA1) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Streptamycin, Tetracycline) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | Yes |
Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli
MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ PIR2 Chemically Competent E. coli
One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ INV110 Chemically Competent E. coli
One Shot INV110 Chemically Competent E. coli cells are derived from the JM110 strain and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ PIR1 Chemically Competent E. coli
One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ BL21(DE3) Chemically Competent E. coli
One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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