Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Only)

Designed for cloning PCR products directly from PCR reaction in 5 minutes

Supplier:  Invitrogen™ K250020SC

Catalog No. K250020SC

Includes: TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli or Mach1-T1^R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes.

Description

• Use pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants
• Comes with Gateway (GW) entry vector, pCR™8/GW/TOPO
• Vector has 3'-T overhangs for direct ligation of Taq-amplified PCR products
• att sites for rapid recombination into variety of Gateway destination vectors
• Novel GW primer sites, within the att regions, located less than 55 base pairs from the PCR product insertion site for convenient sequencing
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

Order Info

Shipping Condition: Dry Ice

Specifications

• Cloning Method: TOPO™ with Gateway™
• No. of Reactions: 20 reactions
• Product Type: TA Cloning Kit
• Vector: pCR

• Content And Storage: The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli or One Shot™ Mach1™-T1^R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes. Store all components of the PureLink™ kit at room temperature. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Product Line: One Shot™
• Shipping Condition: Dry Ice

For Research Use Only. Not for use in diagnostic procedures.